慢性高眼压大鼠外侧膝状体、上丘神经元损伤的研究-眼科学专业毕业论文.docxVIP

青岛大学医学院硕士学位论文CHANGES 青岛大学医学院硕士学位论文 CHANGES oF NEURoUS IN THE LATERAL GENICULATE NUCLEUS AND SUPERIoR CoLUCULUS oF GLAUCoMAToUS RATS 【Abstract】0bjective To investigate neuronal degeneration in the lateral geniculate nucleus(LGN)and superior collieulus(scl and the relationship to optic nerve lOSS in the experimental rat model of glaucoma．Methods Chronic intraoculatpressure elevation was induced by cauterization oftlle limbal vessels in the left eye of 15 rats．The animals were fixed by peffusion from the heart after 2，4，6 months respectively． Histomorphometric analysis was performed on optic nerve and brain cross．sections using bright field microscopy．Both experimental side and control opposite side were studied． Changes of expression of synaptophysin p38 in LGN and SC were studied by immunohistochemistry and estimated by computer image analysis techniques．Results The mean number of neurous in right LGN and SC were significantly decreased compared with control side，Elevation of IOP for 2，4，6 months resulted in reduction of 5％to 23％in the density of LGN and SC neurons mceiving input from the glaucomatous eye．No differential effect in cell density was seen for 2 month group versus 4 month group．nIe values of optic density of p38 immunoreactivity in the right LGN and SC were significantly loweF than that in the left side．Differences m neuron count between control and experimental sides of LGN and SC showed strong association wim differences in optic nerve fibers count and syrmptic denzity．Conclusions The present results suggest that significant lOSS of neurons OCCURS in LGN and SC of tlle experimental glaucomatous rat model，which may result from the lOSS ofoptic nerve fibers． Sun Gang(Ophthalmology) Directed by Wang JinghHa [Key words]glaucoma optic ncrve injuries；Geniculate bodies，Lateral；Superior colliculus；synaptophysin P38；Rat 2 青岛大学医学院硕士学位论文慢性高眼压大鼠外侧膝状体、上丘神经元损伤的研究 青岛大学医学院硕士学位论文 慢性高眼压大鼠外侧膝状体、上丘神经元损伤的研究 研究生孙刚 导师王诤华教授 引 言 青光眼是一组以特征性视神经萎缩和视野缺失为共同特征的疾病。在众多的危险