胍基丁胺对大鼠肺动脉平滑肌细胞增殖的影响及机制的初步研究-病理生理学专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 1 英语缩写一览表 缩写 ACE 英文名称 angiotensin-converting enzyme 中文名称 血管紧张素转换酶 ADC arginine decarboxylase 精氨酸脱羧酶 AGM agmatine 胍基丁胺 Ang-II angiotensin-II 血管紧张素 II DAB diaminobiphenylamine 二氨基联苯胺 HPH 3H-TdR hypoxic pulmonary hypertention 3H-thymidine 缺氧性肺动脉高压 氚标记胸腺嘧啶脱氧核苷 LDH lactate dehydrogenase 乳酸脱氢酶 MAPK mitogen-activated protein kinase 丝裂原活化蛋白激酶 NCS newly-born calf serum 新生小牛血清 NO nitric oxide 一氧化氮 NOS nitric oxide synthase 一氧化氮合酶 NP-40 nonidet P-40 润湿剂 P 40 PASMCs pulmonary artery smooth muscle cells 肺动脉平滑肌细胞 PBS phosphate buffer saline 磷酸盐缓冲液 PCNA proliferating cell nuclear antigen 增殖细胞核抗原 PDGF platelet-derived growth factor 血小板源性生长因子 PI propidium iodide 碘化丙啶 Effect of agmatine on proliferation of rat pulmonary artery smooth muscle cells and its mechanism Abstract Hypoxic pulmonary hypertension plays a key role in the development of high altitude pulmonary edema, high altitude heart disease and chronic pulmonary heart disease. Many recent data indicate that pulmonary vascular contraction, especially pulmonary arteriole smooth muscle, and pulmonary vascular remodeling are the base of maintenance and development of hypoxic pulmonary hypertension. Pulmonary artery smooth muscle cells can sense hypoxia directly. Hypoxia can stimulate proliferation of PASMCs in vitro. Ang-II and PDGF are involved in the mechanism of hypoxia stimulating proliferation of PASMCs. Agmatine, a product derived from the decarboxylation of L-arginine by arginine decarboxylase, has been recently identified in various mammalian organs and tissues. Concentrations of agmatine in different organs and tissues are different. It has been reported that agmatine can inhibit proliferation of many types of cells. The mechanism of agmatine regulating proliferation of PASMCs is not well understood. To determine whether agmatine can inhibit proliferation of rat PASMCs induced by hypoxia and understand the mechanism of agmatine regulating proliferation of