烟草中果胶含量连续流动测定方法的改进-烟草科技.PDF

烟草科技/ Tobacco Science Technology 烟草化学/ Tobacco Chemistry 2014 年第2 期（总第319 期） 烟草中果胶含量连续流动测定方法的改进 1 1 2 1 2 *1 孔浩辉 , 程志颖 , 郭璇华 , 欧阳璐斯 , 胡漫妮 , 沈光林 1. 广东中烟工业有限责任公司, 广州市荔湾区东沙环翠南路88 号 510385 2. 华南理工大学, 广州市天河区五山路381 号 510641 摘要：对烟草果胶含量测定的连续流动法进行了改进，通过酸性醇溶液除杂、弱酸提取和间羟基联苯法 显色，快速测定了烟草中的果胶含量。结果表明：①除杂过程中，组分损失率小于0.2% ；②样品前处理所 需时间仅3 h ；③试样稳定性高，48 h 内检测结果的相对变化率小于1% ；④小分子糖对果胶检测结果的影 响小于0.3% ；⑤方法回收率为96% ～104% 。 关键词：连续流动分析法; 烟草; 果胶 中图分类号：TS411.1 文献标识码：A 文章编号：1002-0861 （2014 ）02-0057-04 Improvement of Continuous Flow Method for Determining Pectin in Tobacco 1 1 2 1 2 * 1 KONG Haohui , CHENG Zhiying , GUO Xuanhua , OUYANG Lusi , HU Manni , and SHEN Guanglin 1. China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510385, China 2. South China University of Technology, Guangzhou 510641, China Abstract: The continuous flow method for determining pectin in tobacco was improved, the pectin in tobacco was rapidly determined following impurity removing with acidic alcohol solution, extracting with weak acid and coloring with m-hydroxydiphenyl method. The results showed that: 1) The loss rate of tobacco component was less than 0.2% during the process of impurity removing. 2) The time required for sample pretreatment was only three hours. 3) The sample was of high stability, the relative variation of the test results was lower than 1% within 48 hours. 4) The influence of small molecular sugars on test results was under 0.3%. 5) The recovery of this method ranged from 96% to 104%. Keywords: Continuous flow method; Tobacco; Pectin ［4-5 ］ 果胶以线性半乳糖醛酸为主，主链分子的基本成 食安全性产生负面影响