蒙药荜茇有效成分衍生物胡椒酸钾抗肿瘤机理生物工程专业论文.docxVIP

优秀毕业论文 精品参考文献资料 内蒙古大学硕士论文ANTITUMOR 内蒙古大学硕士论文 ANTITUMOR MECHANISMS OF POTASSIUM PIPERATE， A DERIVATIVE OF MONGOLIAN MEDICINE Piper longum L． AB STRACT Piper longum L．is a well-known traditional antihyperlipidemie medicine in China， the constituents extracted from the fruit of P longum yielded piperine，pipemonaline， and piperlonguminine．However,the antitumor properties of these constituents liFe not understood at a11．We found that potassium piperate(GBK)，a derivative ofpiperine，has ability to inhibit proliferation of cancer cells，especially mammary cancer cells．It also selectively inhibits the G 1-S—phase transition in mammary cancer cells．Induction of G 1 arrest by GBK is correlated with induction of CDK inhibitor p27 expression as well as inhibition of cyclin E，cyclin A and cyclin B．Moreover’mini．chromosome maintenance(MCM)proteins are markedly down．regulated in MCF．7 cells treated with GBK．On the other hand，GBK selectively kills cancer cells by targeting the stress response to ROS．GBK might also inhibit cancer cell proliferation through epigenetic signaling pathways．Altogether,these results show GBK possesses potential anti． cancer activity against mammary cancer cells by inhibiting cell proliferation and promoting cell apoptosis． KEYWORDS：Mongolian medicine；Piper longum L．；Antitumor mechanisms II 万方数据 内蒙古大学硕士论文目录 内蒙古大学硕士论文 目录 第一章：研究背景概述(文献综述) l 1．1真核生物细胞周期及其调控机制 1 l：1．1真核生物细胞周期概述 1 1．1．2真核生物细胞周期调控 1 1．1．3真核生物DNA复制起始调控 ．2 1．2真核生物细胞凋亡及其调控机制 一5 1．2．1细胞凋亡概述 5 1．2．2 Caspase家族 一6 1．2．3 Caspase激活机制 ．．9 1．2．4细胞凋亡的调控 9 1．2．5细胞凋亡与癌症 ．．10 1．3蒙药荜茇及其衍生物研究进展 ．11 第二章蒙药荜茇有效成分衍生物胡椒酸钾抗肿瘤机理 13 2．1引言 1 3 2．2实验材料 ．15 2．2．1细胞株及培养基 ．15 2．2．2胡椒酸钾 ．．1 6 2．2．3实验试剂 ．17 2．2．4引物 ．．2l 2．3实验方法 22 2．3．1 GBK对正常细胞和癌细胞毒性测定 ．22 2．3。2 GBK作用后细胞活性测定 ．22 2．3．3 GBK药物的选择性抑制作用实验 ．23 2．3．4 GBK与依托泊苷联合用药对细胞活性的的影响 ．23 万方数据 内蒙古大学硕士论文2 内蒙古大学硕士论文 2．3．5细胞克隆形成实验 ．：23 2．3．6 GBK对肿瘤细胞周期的影响 ．24 2．3．7癌细胞中总．RNA提取及Microarry检测 ．25 2．3．8 RNA反转录实验 ．26 2．3．9 Real．Time PCR ．26 2．：；．10 Western Blot ．27 2．3．11 GBK对细胞凋亡的影响 29 2．