关于shh协同骨髓间充质干细胞促进造血干细胞增殖机制的研究内科学专业论文.docxVIP

都高于HSC单独培养组；且HSC+MSC+Shh组高于HSC+MSC组；P值均 都高于HSC单独培养组；且HSC+MSC+Shh组高于HSC+MSC组；P值均 O．05。各培养组MSC的RNA总量：MSC+Shh组、MSC+HSC组都高于MSC 单独培养组；且MSC+HSC+S11h组高于MSC+HSC组；P值均O．05。⑥培 养第7天，利用Q．PCR法分别检钡iJ HSC的Ki67、Tie。2和MSC的VEGF、 AnglmRNA的相对表达量，即RQ值。Ki67的RQ值：HSC+SHH组、 HSC+MSC组都高于HSC单独培养组；且HSC+MSC+S11h组高于HSC+MSC 组；P值均O．05。Tie．2的RQ值：HSC+SHH组、HSC+MSC组都高于HSC 单独培养组；且HSC+MSC+Shh组高于HSC+MSC组；P值均O．05。⑦培 养第7天，各培养组MSC的VEGF的RQ值：MSC+SHH组、MSC+HSC组 都高于MSC单独培养组；且MSC+HSC+Shh组高于MSC+HSC组；P值均 0．05。Angl的RQ值：MSC+SHH组、MSC+HSC组都高于MSC单独培养 组；且MSC+HSC+S1111组高于MSC+HSC组；P值均O．05。结论：1．我们 成功的分离培养出MSC、利用Mini MACS分离装置成功的分离培养出 HSC；2．研究结果发现MSC能够促进HSC的增殖，Shh能够促进HSC的增 殖，且Shh协同MSC促进HSC的增殖，本研究结果与前期研究一致； 3．MSC促进HSC增殖的作用机制与血管生成因子有关；3．Shh促进HSC增殖 的作用机制与血管生成因子有关；4．Shh促进MSC的增殖，其作用机制与 血管生成因子有关；5．Shh协同MSC促进HSC增殖的作用机制与血管生成 因子有关。 关键词：Sonic hedgehog，血管生成因子，造血干细胞，机制，间充质干 细胞 一3- 万方数据 The The synergyism of Sonic hedgehog and bone marrow mesenchymal stem cells for the proliferation of hematopoietic stem cell may be associated with secretion of angiogenesis factor AbstractObjectiveThe in vitro amplification of hematopoietic stem cells (HSCs)Has always been the research hotspot and difficulty．In recent years，many studies have shown that mesenchymal stem cells can promote the proliferation of hematopoietic stem cells．Our previous study in vivo found MSC promote bone marrow proliferation and also found that blood vessel growth significantly，Previous in vitro studies found that the MSC promote HSC proliferation，Shh help MSC promote HSC proliferation．This study will further explore whether MSC promoting HSC proliferation is related to angiogenic factors，and the mechanism of Shh enhancing MSC promoting HSC proliferation．In addition，This study Can not only provide basis for further research of HSC expansion in vitro，but also provide a good application prospect for hematopoietic stem cell transplantation(HSCT)and the therapy of blood system of malignant disease．Method：1．We extract the Healthy volunteers’bone marrow，According to the charact