nos2在小鼠肝损伤中的作用研究生物学细胞生物学专业论文.docxVIP

ABSTRACTNitric ABSTRACT Nitric oxide synthase isozymes has three subtype，NOS 1、NOS2、NOS3，also called nNOS，lNOS，eNOS．Nitric oxide synthase 2，work and produce lots ofnitric oxide when the cell is stimulated and activated．Previous investigation indicated that Nos2 phy a important role in chronic neurodegenerative diseases，inflammation，obstructive diseases，tumour,implantation， damage and resistance to parasitic infections．Although the effect ofNos2 has been investigated in Nos2 knockout young mice，it remained unclear in aged mice． It becomes more and more popular using the gene knockout technology to research gene function．Gene knockout technology has become the hotspot and frontier of biology,and has the profound influence many biological With the increasing of aged population，the number of the old people with hcpatopathy suffering partial liver resection has obvious rising trend．Old people PH had higher mortality and morbidity．NO synthesized by NOS2 plays an important role in the liver regeneration after partial hepatectomy(PH)．During this research，we investigated the effect of liver regeneration after partial hepatectomy in the Nos2-knockout aged mice．2／3 liver resection was performed in 24-mouth·old Nos2-knockout and wild type C57BL／6 mice；liver tissue was collected after PH． The mRNA expression of the Nos2 gene in WT mice was examined by qRT-PCR；the mRNA expression of the immediate early genes Fos and dun，cell cycle related genes Cyclin D1，Cyclin A2，Cyclin BI in both t)Ip伪of mice Was examined by qRT-PCR；the proteins expression of the proinflammatory cytokines TNF-a and IL-6，cell proliferation marker Ki67，cell proliferation III 万方数据 万方数据 万方数据 related related proteins JNK NF-kB，STAT3，apoptosis related proteins CASPASE3，CASPASE9，BCL2 and BAX in both types of mice were examined by Western blot analysis． As compared to WT control，at the termination stage of liver regeneration， Nos2．knockout aged mice had lower survival ratio(P=O．039)and low liver index(from 1 20 hours