多发性骨髓瘤微泡介导骨髓瘤细胞促血管新生活性的新机制-内科学(血液科)专业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 PAGE 3 PAGE 3 组相比明显增加（P0.05）。其中 VEGF 的分泌量在 48h MV 组与 control 组差别不大 （P0.05）。我们进一步探测 MV 促血管新生的机制，激光共聚焦和流式结果显示 MM-MV 可与 EAHY926 细胞直接融合，融合后 CD138+的 EAHY926 细胞占 3.1%； 同时也能与 MM 细胞融合，说明 MV 与靶细胞的融合是具有普遍性，是其主要的作 用方式。MM-MV 与 EAHY926 细胞直接融合而促进 EAHY926 细胞表达分泌 IL-6、 VEGF，进而发挥促血管新生作用。 结论 人 MM-MV 促进肿瘤内皮细胞血管新生，是一种促 MM 血管新生新机制。 关键词 多发性骨髓瘤(MM)；微泡(MV)；内皮细胞(ECs)；血管新生(angiogensis) Mutliple myeloma-derived microvesicles: a novel way to promote myeloma angiogenesis Postgraduate: Liu Yan Supervisor: Chen Zhichao Abstract Objective Angiogenesis plays an essential role in multiple myeloma growth, invasiveness, and metastasis. The specific mechanism of angiogenesis in multiple myeloma (MM) is still unknown. The objective of present study was to investigate the effect of microvesicle (MV) derived from multiple myeloma cells on angiogenesis of endothelial cells (ECs) in the tumor microenvirnment, and to investigate the mechanisms involved. Methods MTT assay, transwell migration assay and tube formation assay were used to study the infection of the Microvesicle (MV) from human multiple myeloma to EAHY926 cells in microenvirnment，as well as RT-polymerase chain reaction (RT-PCR), Enzyme- linked immunosorbent assay (Elisa), Confocal and Flow cytometry methods were employed to study the mechanisms involved. Results Multiple myeloma (RPMI8226)-derived microvesicles promoted EAHY926 cells proliferation, migration and tube formation in vivo and vitro. Confocal and Flow cytometry showed that MM-MV fused with EAHY926 cells. After co-cultured with MM-MV, EAHY926 cells proliferated quickly at 12h, 24h, 36h and 48h (MV vs control group, P0.05). EAHY926 cells in MV group had more migration at 12h, 24h and 36h (P0.05). However, there were no differences in EAHY926 cells migration between MV and control groups at 48h (P0.05). In vitro, there were more tube formation in MV group than control group at 9h and 12h, P0.05); Tube numbers were similar in MV and c