七年制第二讲阿米巴利什曼阴道滴虫ppt课件.ppt

Endemic Areas for Leishmaniasis BMJ 2003;326:378 Morphology Minute elliptical body, 2.9-5.7 μm x 1.8-4.0 μm No free flagellum, Nucleus – deep red, located at one side Cytoplasm-blue, contain: kinetoblast; basal body, rhizoplast 1.Amastigote (Leishman-Donovan? body） 无鞭毛体 kinetoblast Rhizoplast Basal body Nucleus Amastigote Bone morrow smear -- Giemsa stain Blue cytoplasm, red nuclear Morphology Morphology Large numbers of amastigote in a M? M? broken with the release of amastigotes which may invade other M?, start asexual multiplication again Morphology Amastigote Promastigote 前鞭毛体 Spindle shaped with 1 free flagellum Usually gather together in the medium as a mum Morphology Cytoplasm is blue，nuclear is amaranthine（紫红色的） Move actively with the flagellum Morphology Promastigote Life cycle 2-host pattern, mammal ??insect vector Asexual multiplication of amastigote in M? Blood feeding of female phlebotomus, bring amastigote to vector host gut Life cycle Amastigote?Promastigote(3d) ?binary fission to fill the mouth part of sandfly (7d) Transfer of promastigotes to new mammal host by another blood feeding of the insect Promastigote phagositized by M? and convert into amastigote Life cycle M? bring proliferated amastigotes all over the mammal host Dog may serve as a reservoir Amastigotes may directly be transferred by blood transfusion Asexual reproduction takes place in both hosts Life cycle  Phlebotomus (sandfly) Small，about 1/3 of a mosquito, 3mm, yellow-gray Amastigote to promastigote in the insect Life cycle Pathogenesis Compensatory (代偿性的）proliferation of lymph-macrophage system accompanied with auto-immunity Pathogenic factors Host susceptibility Pathogenic effect Humans and dogs are susceptible Rodents are rarely infected Adherence to macrophage (黏附作用) Proliferation and dissemination (繁殖和播散) Inhibition of macrophage apoptosis (抑制巨噬细胞的凋亡) Clinical ma