滤纸条与吸潮纸尖采集龈沟液样本比较.docVIP

滤纸条与吸潮纸尖采集龈沟液样本比较 现代口腔医学杂志2008年第22卷第2期JModemStomatol,March2008,V0l22,No.2?137? 滤纸条与吸潮纸尖采集龈沟液样本比较 陈智滨孙晓军栾庆先 【摘要】目的比较吸潮纸尖与传统滤纸条采集龈沟液样本质和量的差异,为临床选择适宜的采样材料提供 实验依据.方法实验组(A组)吸潮纸尖,对照组(B组)传统滤纸条,手工裁剪成10×2ram长方形.体外实验:质 控血清20个递增体积分别加在两种材料上,同体积重复4次.分析体积与重量之问的关系;检测比较两种材料洗 提液中四种成份回收率差异.体内实验:两种材料问隔三天采集牙周病患者同一位.占’样本龈沟液66份,计算样本 的重量和体积范围,检测比较样本洗提液中的白细胞介素一6(interlenkin一6,lL一6)(n=27),白细胞介素一1B (interleukin—lp,IL—lB)(n=50)含量.结果体外试验结果秩和检验分析两组洗提液中4种成份的回收率gt; 90%,且组间无显着?j生差异(Pgt;0.05).体内实验组问龈沟液净重高度相关关系(r=0.959)且无显着性差异(P gt;0.05).两组材料洗提液中IL—lB和IL一6统计无显着?j生差异Pgt;0.05.结论实验结果证明吸潮纸尖可替代 传统手工裁剪滤纸条,适于国内龈沟液研究工作. 【关键词】龈沟液滤纸条吸潮纸尖 Comparethedi骶rencebetweenthef’dterpaperandabsorbentpaperpointsinconecCmgGCFCHENZhibln,SUN X~ojun,LUANQingxian.DepartmentofPeriodontology,PekingUniversitySchoolandHospitalofSmrm~logy,Beijing lO0o8l 【Abstract】ObjectiveTocomparethedifferenceofabsorbentpaperpointsandwhatman3stripfilterpaperin collectingGCFandaimofselectingthesuitablesamplingmateria1.MethodsAbsorbentpaperpointswereusedas experimentalgroupandwhatman3stripfdterpaperascontrolgroup.Thepapemwerecutinto10×2mmrectangle manually.Invitrostudy.TheSerlllnwasaddedontothetwomaterialstlIincrementalvolume.Theprocedurewas repeatedfourtimesWitlIthesamevolume.Therelationshipbetweentheweightandvolume,aswellaSthedifferenceamong recoveryratesoffourcomponentsintheelutionwereanalyzed.Invivostudy,GCFsamplesfromperiodontalpatientswere collectedwith3daysintervalbyusingthetwomaterialsrespectively.Theweightandvolumeofthesampleswerecalculated andcontentsoftheinterleukin-6(IL一6)andtheinterleukin一113(IL一113)intheelutionwerecompared.ResultsIn vitrostudy,nostatisticallysignificantdifference(Pgt;0.05)wasshownbetweenthetwogroupsintherecoveryratesofthe fourcomponents.Invlvostudy,nostatisticallysignificantdifference(Pgt;0.05)WaSshownbetweentwogroupsinGCF weightandcontentsofIL一6andIL一113intheelutlon.Highcorrelation(r=0.959)wasfoundbetweentwogroupsin GCFweight.ConclusionThepaperpointsCanbeapedasaneasyandaccuratesamplingmethodwhichmaybea substituteforwh