12联合IL2在伯氏疟原虫红内期感染中的免疫调节作用.docVIP

12联合IL-2在伯氏疟原虫红内期感染中的免疫调节作用 ? 734? 【文章编号】1672—3619(2005)06-0734-04 — o— u— rn— al— o— fTropicalMedicineVo1.5No.6Dec.2005 ? 论着? IL-12联合IL-2在伯氏疟原虫红内期感染中的免疫调节作用 陈妹,陆惠民,张山鹰:,高琪s,唐学恒? (1.苏州大学医学院寄生虫学教研室,苏州215007: 2.福建省寄生虫病防治研究所,福州214064; 3.江苏省寄生虫病防治研究所,无锡350001) 【摘要】目的探讨IL.12联合IL.2在抗只berghei红内期感染中的免疫调节作用.方法BALB/c小鼠 接种5xl0s个感染P.berghei的RBC,同时分别给予0.03g/dIL.12,40U/dIL.2或IL.12联合IL.2处理,连续 用药6d,观察小鼠原虫血症变化及存活时间.通过淋巴细胞增殖转化试验,T淋巴细胞亚群测定及NK细胞杀 伤活性检测等方法,观察细胞免疫应答水平.结果IL.12联合IL2处理组可较单独IL.12或单独IL.2及感染 对照组显着推迟原虫血症达峰值的时间与明显延长小鼠的存活时间,IL.12或联合IL.2组小鼠在原虫血症达 30%时开始死亡,而单独IL.2或感染对照组小鼠在原虫血症低于13%时已全部死亡.IL.12联合IL.2处理组 脾淋巴细胞总数,CD4与CD8百分比,稍.TdR掺入量及NK细胞杀伤活性均较其它各处理组及感染对照组显 着升高.结论IL12与IL.2二者可协同促进脾淋巴细胞的增殖及增强NK细胞杀伤活性以诱导抗P.berghei红 内期感染的免疫保护作用. 【关键词】IL.12;IL.2;伯氏疟原虫;红内期;免疫调节 中图分类号:R382.31文献标识码:A RegulationoftheImmuneResponsestoBlood?Stage P.bergheiInfectionbytheCombineTreatmentwith几-12and几-2 CHENShu,LUHui—min,ZHANGShan—ying,GAOQi,TANGXue—heng (DepartmentofParasitology,SchoolofMedicine,SuzhouUniversity,Suzhou215007,China) 【Abstract】ObjectiveTostudythecombinedeffectsofIL-12andIL-2onthedevelopmentofprotective immunitytoblood-stageP.berghei.MethodsP.bergheiinfectedBALB/emiceweretreatedwith0.03g0fIL一12 aloneorincombinationwith40UofIL一2onceadayforsixconsecutivedays.Parasitemiawasmonitoredandsarvival timeWascalculated.Cellularimmuneresponseswereelvaulatedbylymphocyteproliferationtest.CD4+andCD8Tcells coun~andNKcellcytotoxieity.ResultsCombinedtreatmentwithIL.12andIL-2COUldsign/fieantlydelayedthepeakof parasitemiaandprolongedSlIF9ivaltimeoftheP.bergheiinfeetedmicewhencomparedwiththetreatmentwithIL.12or IL.2alone.Moreover.thesurvivalshipoftheinfeetedmicetreatedwithIL.12aloneorwithacominationofIL.12and IL.2startedtodropat30%parasitemia.whereasmicetreatedwithIL.2aloneorwithouttreatmentalldiedbefore reached13%ofparasitemia.Further.micereceivedthecombinetreatmentwithIL.12andIL.2showedsignificant increasesinthenumbersofspleniclymphocytes,thepercentagesofCD4andCD8Tcells,thelevelsofH桾dR incorporationofspleniccellsandtllea