不同来源沙门氏菌分离鉴定及其耐药谱型比较.docVIP

不同来源沙门氏菌分离鉴定及其耐药谱型比较 　　摘要：利用亚硒酸盐胱氨酸增菌液（SC）、沙门氏-志贺氏属琼脂培养基（SS）、科玛嘉沙门氏菌显色培养基、Reveal沙门氏菌检测试剂盒和分子生物学方法对不同动物的粪便样本进行沙门氏菌（Salmonella）的分离和鉴定。采用微量肉汤稀释法对分离菌株进行恩诺沙星、安普霉素和氟苯尼考等17种抗菌药物敏感性试验，试验数据采用 Whonet5.4软件统计以比较不同来源沙门氏菌的分离率和耐药谱型差异。结果表明，不同动物来源沙门氏菌的分离率和耐药谱型差异较大，其中牦牛源沙门氏菌的分离率和耐药率最低，禽源和犬源沙门氏菌的分离率差别不大，但耐药谱型差异较大。 　　关键词：沙门氏菌（Salmonella）；分离鉴定；耐药谱型 　　中图分类号：S852.61 文献标识码：A 文章编号：0439-8114（2012）21-4834-03 　　Isolation， Identification and Drug Resistance Spectrum Comparison of Salmonella from Different Sources 　　CHEN Chao-xi1，DU Xiong-wei2，SHI Zhi-hai3 　　（1. College of Life Science and Technology， Southwest University for Nationalities， Chengdu 610041，China； 　　2. College of Life Science and Technology， Dalian Nationalities University， Dalian 116600， Liaoning，China； 　　3. Insititute of Animal Husbandry and Veterinary Research， Henan Academy of Agricultural Science， Zhengzhou 450002，China） 　　Abstract： The Salmonella from manure samples of different animals were isolated and identified by use of selenite cystine enrichment broth（SC）， Salmonella-shigella agar（SS）， CHROM agar Salmonella colored medium， the Reveal Salmonella detection kit and molecular biological method. Microdilution broth method and Whonet5.4 software were used for antimicrobial susceptibility testing of the isolated strain to Enrofloxacin， Apramycin， Florfenicol and other 14 kinds of antimicrobial agents and the analysis on the isolation rates and drug resistance spectum in different sources of Salmonella. The results showed that there existed apparent differences in Salmonella isolation rates and drug resistance patterns from different animal origins. The isolation rate and drug resistance rate in Yak origin Salmonella were the lowest. The isolation rate between the Salmonella in avian and canine origin had no significant difference， but the drug resistance spectrum showed significant difference. 　　Key words： Salmonella； isolation and identification； drug resistance spectrum 　　沙门氏菌（Salmonella）是一种重要的人畜共患病原菌，其耐药性问题日益严重，在抗生素持续压力