Tenminute DNA Release Kits ature and Science十分钟的DNA释放包自然科学.docVIP

Ten-minute DNA Release Kits - A novel approach to obtain DNA easily in modern biological science Huaijie Zhu *, **, Yucui Zhu *, **, Hongbao Ma ***, Jiesheng Lu * * Jacksun Easy Biotech Inc., New York, NY, USA, hjz689@; ** Columbia University, New York, NY, USA, hz42@ *** Michigan State University, East Lansing, MI, USA, hongbao@ Abstract: Genomic DNA study has been used widely in biology, genetics, reproduction, clinical medicine and clinical medicolegal. This article describes characterization and protocol of the new product 10-minute DNA Release Kit that provides a reliable, simple, and quick approach, by taking 10 minutes at 86?C for DNA release and desired DNA extract obtaining. The product will save scientists’ time and give better result on the DNA extract. [Nature and Science. 2006;4(2):58-70]. Keywords: DNA; extract; knowledge; PCR 1. Introduction Genomic DNA study has been used widely in biology, genetics, reproduction, clinical medicine and clinical medicolegal. It is very important for the genomics study to obtain the desired DNA extract easily and quickly. The 10-minute DNA Release Kit provides a reliable, simple, and quick approach, by taking 10 minutes at 86?C for DNA release and desired DNA extract obtaining. The 10-minute DNA Release Kit not only offers a wide range on the desired DNA extract (Figure 1), but also gives a good expression of multi-primers in polymerase chain reaction (PCR) (Figure 2). And, it is not only used for on genome expression by the analysis of PCR, but also used on mitochondrial DNA mutation for the diagnosis and treatment observation of mitochondrial disease, which can be done by the analysis of PCR/RFLP (Restriction Fragment Length Polymorphism) and point mutation (Figure 3).  Figure 3. Primer expression on DNA volume-dependent effective with the DNA extract (1) Primer, Gapdh and Cyclin D1 are designed from human Genomic DNA Sequence. Gapdh Start Sequence