Mining for metods of gene regulation H region of Mor28对mor28基因调控区的方法挖掘.pptVIP

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Mining for metods of gene regulation H region of Mor28对mor28基因调控区的方法挖掘.ppt

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Mining for metods of gene regulation H region of Mor28对mor28基因调控区的方法挖掘

A Computational Analysis of the H Region of Mouse Olfactory Receptor Locus 28 Deanna Mendez SoCalBSI August 2004 Overview Background of olfactory receptors Introduce the H region as a possible cis regulatory element Define cis regulatory elements Results of looking for the H region Discussion of other possibile explanations of H Future work Acknowledgements References Background of Olfactory Receptors A single odorant receptor gene is expressed by an olfactory neuron and that the axonal projections map to different glomeruli on the olfactory bulb. In 2003 a region of homology called the H region was identified as a putative cis regulatory element of the a particular set of olfactory receptors – MOR28 and their orthologous counterparts in humans. Sample Olfactory Receptor Locus: Mor28 Mouse and Human Model Conserved 1.6kb region Without the H region there is little or no expression of ORs Cis acting elements A Cis acting elements are a sequence of DNA that can bind transcription factors and in so doing, control or modulate the level of transcriptional initiation from one or more nearby genes.6 BLAST and Blat whole genome view across genome view Family Relations local view of DNA Dot Plot: Human and Mouse Pair view of Mouse and Human H region aligned at a 85% threshold Dot Plot 95% similiarity Need for a node in the evolutionary Tree to obtain more general information Dog Genome was released, assembled, and made publically available in July 2004. To find an orthologous region in dog I used the three known olfactory receptors in mouse and blasted them to the dog genome and then looked for the gene upstream of the H region in mouse in the dog genome. The BLAST result of Mor28 Locus on the Dog Genome Orthologous Mor28 site in Dog 2kb H region of mouse BLASTED on the dog genome 300bp H region from Mouse(32) on Dog Three way of comparison of Mouse, Human, and Dog Another possibility Future Work Acknowledgments Barbara Wold for giving me the opportunity to work in her lab