烟草烟雾对大鼠牙槽骨内牙本质基质蛋白1C末端表达影响.docVIP

烟草烟雾对大鼠牙槽骨内牙本质基质蛋白1C末端表达影响 　　[摘要]目的观察烟草烟雾对大鼠牙槽骨内牙本质基质蛋白（DMP）1 C末端表达的影响。方法建立试验大鼠吸入烟草烟雾模型，免疫组织化学法检测DMP1 C末端在大鼠牙槽骨内的表达。结果阳性对照组：DMP1 C末端深棕黄色连续线形表达于固有牙槽骨的钙化前缘，形成明显的矿化条带；棕黄色颗粒状密布于骨细胞周围，浅棕黄色颗粒状散布于矿化区域；上述表达由牙槽嵴顶至根尖方向逐渐上调。试验组：DMP1 C末端表达部位同对照组，表达强度下调且随试验时间的延长下调加重。结论吸入烟草烟雾可下调大鼠牙槽骨内DMP1 C末端的表达；在试验周期内，DMP1 C末端的表达下调呈时间依赖性。 　　[关键词]烟草烟雾；牙槽骨；矿化；牙本质基质蛋白 　　[中图分类号]Q 786[文献标志码]A[doi]10.3969/j.issn.1673-5749.2012.04.002 　　The effect of tobacco smoke on the expression of the dentin matrix protein 1 C-terminal in rat alveolar boneHou Xiumin1, Ma Su1, Liu Peihong1, Qin Chunlin2, Chen Li2.（1. Dept. of Periodontics, Hospital of Stomatology, Harbin Medical University, Harbin 150001, China；2. Heilongjiang Scholars Laboratory, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China） 　　[Abstract]ObjectiveTo observe the effect of tobacco smoke on the expression of the dentin matrix protein（DMP）1 C-terminal in rat alveolar bone. MethodsThe model of the test rats inhaled tobacco smoke was established. The expression of the DMP1 C-terminal in the alveolar bone was detected by immunohistochemistry. ResultsControl group: DMP1 C-terminal continuous linear expressed in the calcification front of the proper alveolar bone and it is dark brown yellow, apparent mineralization strap was formed; DMP1 C-terminal brown granular dense clouded around the bone cells and light brown yellow fine granules scattered in the mineralized zone; the expression of the DMP1 C-terminal gradually increased from the alveolar ridge crest to root tip. Experimental group: DMP1 C-terminal parts of the same expression in the control group, decreased intensity of expression, this change increased with prolonged test time. ConclusionInhalation of tobacco smoke can decrease the expression of the DMP1 C-terminal in rat alveolar bone. The effect presents time dependent in this study period. 　　[Key words]tobacco smoke；alveolar bone；mineralization；dentin matrix protein 　　吸烟是牙周疾病一项重要的危险因素[1]。长期暴露于烟草烟雾中的大鼠牙槽骨矿