ibp在口腔鳞癌中的表达及增殖作用分析-expression and proliferation of ibp in oral squamous cell carcinoma.docx

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 4 We examined IBP expression in OSCC specimens and normal oral mucosa epithelium tissues by Immunofluorescence staining. It was indicated that high expression of IBP can be detected in some of OSCC specimens, but not in normal oral mucosa epithelium tissues. In addition, IBP expression patterns are determined in OSCC cancer by double immunofluorescence staining. The results show that IBP expression patterns were primarily in the cytoplasm. IBP expression was detected by IHC with anti-IBP polyclonal antibodies in 78 primary OSCC cancer tissues, as well as 6 normal oral mucosa epithelium tissues. The results indicated that IBP was negatively expressed in all of 6 normal oral mucosa epithelium tissues; whereas IBP was overexpressed in 36 OSCC tissue samples (46.15%). Fisher’s exact tests were performed to evaluate the IBP expression in different OSCC specimens. A value of p 0.05 was regarded as statistically significant. IBP expression significant positively correlated with tumor size (p = 0.008), clinical stage (p = 0.024), and distant metastasis (p = 0.034), but significant negatively correlated with differentiation (p = 0.007). IBP promotes cell proliferation in oral squamous cell carcinoma We have studied IBP expression in Tca8113, MDA-MB-231, MDA-MB-231 /SR119-2, MDA-MB-231/con on WB. The construction of IBP over-expression model by plasmid transfection was based on IBP negative expression cell line- Tca8113. The MTT assay was used to determine the effects of IBP on cell proliferation in each group. In our study we found that up-regulation of IBP expression greatly promoted the proliferation of OSCC cells in MTT assay compared with that in control groups. And down-regulation of IBP expression greatly inhibited the proliferation of MDA-MB-231 cells in MTT assay compared with that in control groups. Colony formation assay was used to determine the effects of IBP on cell growth in OSCC cells. It was shown that overexpre