hsp70上调对快速心房起搏致兔房颤离子通道重构影响-effect of hsp 70 upregulation on ion channel remodeling in rabbit atrial fibrillation induced by rapid atrial pacing.docx

新疆医科大学硕士学位论文and after pacing. (3) the inducibility of atrial fibrillation in each group were by programmed and burst stimulation.The the inducibility of atrial fibrillation of sham groups were 25% at each time point;The the inducibility of atrial fibrillation of pacing groups respectively were 37.5%,75%,100%,100%at pacing 0h,2h,4h,6h.Them were respective 25%,37.5%,37.5%,50% in heat stress groups;Them were respective 37.5%,50%,50%,62.5% inatorvastatin group, It did not significant change during the 6h of pacing in sham groups. (4)The expression of HSP70 and HSP70mRNA of all parts ofthe heart in Heat stress group and the atorvastatin group was significantly higher (P ＜0.05). There was no significant changes between pacing group and the sham group. The expression of Cav1.2,α1c,mRNA,Cav1.2,α1c and KCa3.1mRNA,KCa3.1 protein in pacing group was decreased compared with heat stress group and the sham group.But it had no statistical difference among heat stress group, A asked atorvastatin group and the shamgroup. Conclusions: (1) heat stress to rabbit maintain rectal temperature at 41 ℃ for15min induced upregulation of atrial expression of HSP70, high expression of HSP70 was able to reduce of inducement of atrial electrical remodeling and AF by atrial pacing significantly; (2) Atorvastatin increased the expression of HSP70mRNA in myocardial, and it had significant preventive role to myocardial electrical remodeling and AF induced by atrial pacing; (3) the Expression of HSP70 is an important factor which can influence atrial ER and AF; (4) the upregulation of HSP70 in Myocardial inhibited rapid atrial pacing induced reconstruction of Cav1.2,α1c and KCa3.1; (5) Atorvastatin suppressed rapid atrial pacing inducd Reconstruction of Cav1.2,α1c and KCa3.1 via increasing HSP70 expression; (6) Preventative effect of HSP70 high expression to atrial ER and AF maintained 6 h.Keywords：heat shock; heat shock protein 70;atorvastatin;cardiac pacing; Cav1.2,α1c; atrial fibrillation; electica