atra增强hsvtkgcv对膀胱癌旁观者效应的实验研究word格式论文.docxVIP

英文缩写一览表英文缩写ATRA英 文 全 称All trans retinoic acid中文全称全反式维甲酸DMSO DEPC 5’ -LTR EDTAPBSDimethyl sulfoxide Diethyl pyrocarbonate 5’ -long terminal repeatEthylendiaminotetracetic acidPhosphate buffer saline二甲基亚砜 焦碳酸二乙酯 5’ 长末端序列 乙二安四乙酸磷酸缓冲生理盐水LB TERT-PCRP-gp KDE.coli DOTAP EBO.DH .B d NTP NBT DAB MTTLuria bertani Tris –Hcl EDTARevert-transcription poly-chain reaction P-glycoproteinKilo dulun Escgerichia ColiN,N,N,-trimethylammonium methyl sulfate Ethidium bromideOpical density Hygromycin BDeoxybonucleotide triphosphate Nitrio blue tetrazoliu chloride diaminobezidin3’ -(4,5-dimethyl-2-thiazoly)2,5-diphenyl-LB 培养基 TE 缓冲液 反转录 PCR 糖蛋白 千道尔顿 大肠杆菌 脂质体 溴化乙锭 光密度 潮霉素 B寡脱氧三磷酸苷 硝基四氮唑蓝 二氨基联苯氨四甲基偶氮唑蓝2H-tetrazolium bromideCx 26Connexin 26缝隙连接蛋白 26 GJICGap junction intercellular communication缝隙连接细胞间通讯 HEHematoxylin and eosin staining苏木素依红染色SABCStreptavidin-biotin-enzyme complex链霉素亲合素-生物素复合物TUNEL(TDT)Terminaldeoxynucleotidyltransferase mediated X d UPT nick end labeling末端脱氧核苷酸转化酶SLDYScrap loading and dye transfer划痕标记示踪技术LYLucifer yellowCH罗氏黄荧光染料HSV-TKHerpes simplex virusthymidinekinase单纯疱疹病毒胸苷激酶GCVganciclovir丙氧鸟苷RNAaseribonuclease核酸酶L PLog phase对数期Triton-100Poctyl polyethylene glycol phenylether聚乙二醇辛基苯基醚Study of ATRA Enhancing the Bystander Effect of HSV-TK/GCV Gene System on Bladder CancerAbstractBackground: Bladder cancer is one of the most common malignant in urinal system. The curative effect is reduced for the high recur rate after surgery and unsensitivity to the chemical therapy. It is one of the most important strategies of gene therapies to transfect HSV-TK into urinal bladder cancers at present, which shows preferable research value and clinical applying foreground. Many studies have confirmed that the “bystander effect”, that the positive-affected cells can induce the negative-affected cells apoptosis is an important approach. It and the efficiency of transfection are regarded as the two key elements of successful suicide gene therapy. More and more attention was paid to the bystan