医学微生物学--临床螺旋体.pptVIP

梅毒螺旋体 Treponema pallidum 生物学性状 0.1～0.2 × 6～15um; 8～14 小而规则的螺旋，两端尖直。 1.形态 Histology: Treponema pallidum grow in Rabbit’s testis Brown color stained with Fontana silver impregnation stain 2.培养特点 △ 目前暂不能在无生命人工培养基中培养 ※ Nichols strain: virulent, cultured in rabbit testis or anterior chamber of eye, slow growth (doubling time is 30 hr), if cultured in rabbit testis fragments containing amino acids, the virulence will be lost and Nichols strain become to Reiter strain. ※ Reiter strain: non-virulent, infected rabbit testis fragments. △ TP can cultured in cotton tail rabbit epithelial cell and maintain its virulence 抵 抗 力 1. 流行病学特点 Pathogenicity and Immunity △ 人类是唯一的自然宿主 △ 传播方式： ※ 性接触传播(sexual contact) ※ 母婴传播(congenitally) ※ 输血传播(blood transfusion) △ 荚膜样物质(Capsule-like material) △ 外膜蛋白(promote adherence to host cell) △ 透明质酸酶(facilitate perivascular infiltration) △ 纤维连接蛋白(against phagocytosis) △ 病理性体液或细胞免疫反应 2. Virulence factors 3. 所致疾病 △ 获得性梅毒（Acquired syphilis） ※ 性病梅毒（venereal syphilis ） ※ 输血梅毒（syphilis caused by blood transfusion） △ 生天性梅毒（Congenital syphilis） 性病梅毒（venereal syphilis ） Primary stage ※ 3 weeks after infection ※ primary lesion —hard chancre ※ rich in TP ※ infectious highly ※ 4~8w disease course ※ subside spontaneously Secondary stage △ 2~10w after primary stage ※ systemic spread ※ many organisms ※ skin, particularly △ flu-like symptoms, rash anywhere on the body △ rich in TP △ infectious highly △ 3w~3m disease course △ subside spontaneously △ 5~10 years after infection △ granulomatous lesions in skin，bone, and liver △ degenerative changes in the central nervous △ cardiovascular lesions △ few TP in lesion and blood Tertiary stage Acquired Syphilis Primary and secondary syphilis also called early syphilis. △ infectious highly △ destructive small Tertiary syphilis also called lat