苦参碱和氧化苦参碱的提取分离鉴定.pptVIP

4. Procedures for Exp. 1 It is important to ensure that the glass plates are clean and free grease before use. Weigh 10 g silica H and 30 ml CMC-Na (0.8%) , and mix smoothly in a 50 ml beaker. Spread the slurry quickly on to the plates from one to another, and then shake the plates to form a uniform film. Transfer the TLC plates to an even plane and dry it in the shade. Activate the TLC plates at 105oC in an oven for 30 minutes. Allow the TLC plates to cool to room temperature and store them in a desiccator. 4. Procedures Extraction of matrine and Oxymatrine from S. flavecens. 300 g×2 (2 groups) crude slices of S.flavescens was extracted with 0.5% HCl (3000 ml) for 1 day at room temperature→Add 0.1% sodium benzoate (3g)→Repeat the above procedure once again →combine the twice extract. Resin Preparation 150 g cation exchange resin was soaked in 2 M HCl for 45 min→Wash to pH 5-7 with water→Soak the resin with 5% NaOH for 45 min→Wash to pH 7 with water→ Soak the resin with 2 M HCl for 45 min→Wash to pH 7 with water → store in 500 ml beaker with water and label name. 4. Procedures (3) Exchange of the acidic solution and precipitation reactions of the alkaloids. a. The extraction was filtered and passed through the prepared resin（flow rate) (the exchanging end point was detected by dragendorff reagent) →Wash the resin to pH 5-7 with water→Then the resin was vacuum filtered and put into a paper box for open-air drying and label name. 4. Procedures (3) Exchange of the acidic solution and precipitation reactions of the alkaloids. b. The plant extracts were tested for the presence of alkaloids with alkaloid-precipitating reactions. The precipitation reagents include dragendorff reagent 碘化铋钾, silicotungstic acid硅钨酸, tannic acid鞣酸, picric acid苦味酸 and iodine-potassium iodide (碘-碘化钾）. 来后打开水浴锅开关 仪器干燥（包括展开缸） Add 20 ml (湿润即可) concentrated NH3. H2O to the resin 4. Procedures (5) Detection of Matrine and Oxymatrine.