坛紫菜分类学研究.docxVIP

坛紫菜(Pyropia haitanensis)核糖体基因簇序列的测定张皓 何渊 沈颂东苏州大学医学部基础医学与生物科学学院 邮编：215123作者简介：张皓（1985-），男，江苏太仓人，硕士，主要研究方向为基层医学与养殖E-mail：richard HYPERLINK mailto:02@163.com 02@163.com何渊（1990-），男，江苏太仓人，硕士，主要从事藻类细胞发育与分子机理研究。E-mail：756072102@。通信作者：沈颂东（1968-），男，博士，教授，主要从事藻类细胞发育与分子机理研究。E-mail：shensongdong@摘要：坛紫菜是我国独有的栽培紫菜物种，而目前对坛紫菜分类学层面上仍缺乏有效的分子鉴定技术。本研究通过PCR方法，对采自福建省莆田海区坛紫菜样品核糖体基因簇序列进行了测定，目的是为坛紫菜分类及种质鉴定提供分子生物学证据。测定结果表明，坛紫菜样品的18S rDNA的全长序列长度为2953bp；28S rDNA部分序列3603bp，且不含内含子；5.8S rDNA长度为158bp，该区域的5’端和3’端十分保守，在紫菜属中差异较小；ITS1的长度为334bp；ITS2为678bp。已经测定IGS区的部分长度为3555bp，该区域存在复杂二级结构。通过对坛紫菜核糖体基因簇序列的分析表明，其能够成为紫菜分类鉴定与系统演化的分子标记。关键词：坛紫菜；核糖体；顺反子；系统演化The nuclear ribosomal DNA (nrDNA) cistron sequence of Pyropia haitanensis (Bangiales, Rhodophyta)Hao Zhang, Yuan He, Songdong ShenSchool of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, ChinaAbstract: Pyropia haitanensis is a unique cultivated laver in China, while the lack of effective molecular idenfitication technology affects the taxonomy of P. haitanensis. The study sequenced the nuclear ribosomal DNA (nrDNA) cistron sequence of P. haitanensis from Putian, Fujiang Province by polymerase chain reaction (PCR), in order to provide molecular biological evidience for classification and germplasm identification of P. haitanensis. Results shown that each unit is composed of the small-subunit (SSU) rRNA gene of which the full length is 2953bp; the partial sequence of the large-subunit (LSU) rRNA gene of which the length is 3603bp without any introns; the length of the 5.8S rRNA gene is 158bp, the 5’ and 3’ terminal sequences of the genic regions of the ribosomal cistron are extremely conservative among genus of Pyropia; the length of ITS1 is 334bp and ITS2 is 678bp; the partial sequence of IGS is 3555bp, due to a complicated secondary structure exsits in IGS region makes sequencing untoward. Though the analysis of nuclear ribosomal DNA sequence, it can be a molecular maker for the taxonomic identification of Pyropia.Keywords Pyropia ha