产纤溶酶菌株的分离筛选.pdfVIP

31 1 Vol. 31 No. 1 2 0 0 8 1 JO U RN AL O F AG RICU LT U RAL U N IV ERSIT Y O F H EBE I Jan . 2 0 0 8 : 1000 - 1573( 200 8) 01- 0056- 04 郑丽伟, 于宏伟, 贾英民 ( , 07 1001) : , , , , 40 , 2 , ZL W - 2, , , ZL W- 2 , : ; ; : Q 933 : A Screening of fibrinolytic enzymeproducing strain ZHENG Liwei, YU Hongwei, JIA Yingmin ( College of Food Sc ence and T echnology , Agr cultur al U n v ers ty of Hebe , Baod ng 07100 1, Ch na) Abstract: Forty f br nolyt c enzymeproduc ng stra ns w ere solat ed f rom shambles , sew age farm and so on, us ng the soluble c rcle as screen ng marker . T he basal med um w t h sk m m lk was used as the f rst screen ng med um and t he f br n pow der w as used as t he second screen ng med um. St ra n 2 wh ch had t he h ghest f br nolyt c enzym e w as chosen and numbered ZL W - 2. Moreover, t he met hod of measur ng enzyme act v ty w as renovat ed and t he curve of enzy me product on w as pr mar ly stud ed. Ultrav olet phot omet ry w as adopted t o measure enzym at c act v t y n t he exper ment, wh ch had mproved accuracy of t he measurement and had great s gn f cance f or mprov ng eff c ency of screen ng st ra ns produc ng f br noly t c enzyme. T he fermentat on process of ZL W- 2 nd cat ed that t he act v t y of f br nolyt c enzyme ncreased aft er m ass grow th of t hal . Key words: f br nolyt c enzyme; st ra n ; screen ng [ 1] , , [ 4] ,