肥大细胞组胺体外释放模型在食品过敏原分析中的应用.PDFVIP

576 2008, Vol. 29, No. 08 食品科学 ※营养卫生 刘婉莹，向军俭* ，蒋红玲，王 宏，唐 勇 (暨南大学分子免疫学与抗体工程中心，广东广州 510632) ：目的：应用本室的肥大细胞组胺体外释放模型，分析食品过敏原中不同致敏组分的致敏差异性，验证 建立的过敏原体外评价新方法。方法：牛奶、虾粗提物分别免疫C57/BL6 小鼠，收集小鼠腹腔致敏肥大细胞 (PMC) ，分别用牛奶、虾不同过敏组分诱导PMC 释放组胺，荧光法检测其释放水平。结果：牛奶中酪蛋白诱 导牛奶致敏PMC 释放组胺最多，脱脂乳和乳清次之，α-La 则最少。酪蛋白是牛奶致敏蛋白中主要组分。虾致 敏蛋白中，以80kD 蛋白诱导组胺最多，36kD 蛋白次之，21kD 蛋白最弱，虾致敏蛋白混合诱导明显比单独诱导 释放组胺多，说明80kD 蛋白在虾致敏蛋白中起主要作用，并且各致敏蛋白间存在协同效应。结论：肥大细胞体 外组胺释放量与过敏原种类及量存在相关性，本研究为进一步建立研究食品过敏和食品过敏原的新模型系统奠定基 础。 ：肥大细胞；细胞模型；组胺； 食品过敏原 Cell Model of Releasing Histamine by Sensitized Mast Cells in vitro Used for Analysis of Food Allergens LIU Wan-ying ，XIANG Jun-jian* ，JIANG Hong-ling ，WANG Hong ，TANG Yong (Molecule Immunology and Antibody Engineering Center, Jinan University, Guangzhou 510632, China) Abstract：Objective: In this study, a cell model of releasing histamine by sensitized mast cells in vitro was established to analysis the irritability of different allergens in food. Methods : The mice were sensitized by milk and shrimp, respectively. Peritoneal mast cells (PMC) were collected and then were induced to release histamine with various kinds of allergens. Histamine was detected using fluorescence. Results: The primary allergens in milk can induce histamine release from sensitized-mast cells, while casein has the strongest sensitization effect and α-La the weakest. Casein plays a pivotal role in milk. The 80 kD protein has the strongest sensitization effect, followed by 36 kD and 21 kD. The mixture of shrimp allergens can induce stronger histamine release from the mast cells than shrimp allergen alone, implying that there may be some synergistically effects among those different allergens. The 80 kD protein plays a pivotal role in shrimp-induced sensitizatio