改良穿刺法建立大鼠无血预充非停跳体外循环后认知功能障碍模型.pdfVIP

中国体外循环杂志2017年09月15 日第 15卷第3期 ChinJ ECC Vol.15 No. 3 September 15，2017 179 ·基础研究 · DOI：10.13498／ ki.chin.j.ecc.2017.03.14 改良穿刺法建立大鼠无血预充非停跳 体外循环后认知功能障碍模型 段铭杰，孙莹杰，郑　 翔，李思沁，屈鹏霞 ［摘要］：目的　 寻找建立大鼠无血预充非停跳体外循环术后认知功能障碍模型的简便方法。 方法　 SD大鼠20 只，0.2％ 戊巴比妥钠溶液腹腔注射麻醉。 经由锁骨上入路右上腔静脉穿刺引流，尾动脉穿刺灌注，隐动脉穿刺监测血压。 术中持续监 测心率、血氧饱和度及肛温。 转流流量维持在 100 ml／ （min ·kg），转流中根据血气结果适当补充葡萄糖酸钙、潘南金（门冬氨 酸钾镁注射液）和碳酸氢钠调整血气指标，体外循环转流60 min。 结果　 16只大鼠成功存活，存活大鼠术后一天均可完成水 迷宫训练。 结论　 此种改良穿刺方法建立的体外循环模型，创伤小、简便易行，可用于术后行为学检测。 ［关键词］：　 静脉穿刺；认知功能障碍；体外循环；水迷宫 Establishment of rats＇ cardiopulmonary bypass POCD model by meliorated puncture method － － － － Duan Ming jie，Sun Ying jie，Zheng Xiang，Li Si qin，Qu Peng xia Department of Anaesthesiology，General Hospital of Shenyang Military Region，Shenyang 110016，China － Corresponding author：Sun Ying jie，Email：sunyingjie9＠ ［Abstract］：Objective　 To explore a convenient method of cardiopulmonary bypass POCD model in rats. Methods　 Twenty Sprague Dawley ratswere anesthetized with Pelltobarbitalum Natricum i.p. Right upper vena cava was punctured for the drainage of blood and caudal artery for perfusion. Heart rate，anal temperature and blood oxygen saturationwere monitored. Meanwhile，the blood gas indexeswere measured and adjusted duringtheoperation.Theflow of CPBwasmaintained 100 ml／ （min ·kg）andtime interval of CPBlastedfor60mins.Results　 16modelswereestablishedsuccessfully andall ofwhichwereabletocompletethewater mazetrain⁃ ing the day after operation. Conclusion　 The CPB model established by the improved puncture method can shorten the operation time and reduce the difficulty of operation with more convenience and less trauma. ［Key words］：　 Vein puncture；Pocd；Cardiopulmonary bypass；Water maze 　 　 研究表明体外循环（cardiopulmonary by