丁苯酞对血管性痴呆大鼠海马组织tnf―α和il―1β表达的影响.docVIP

丁苯酞对血管性痴呆大鼠海马组织TNF―α和IL―1β表达的影响 　　[摘要] 目的 观察丁苯酞（NBP）对不同时间点血管性痴呆大鼠海马组织肿瘤坏死因子α（TNF-α）和白介素1β（IL-1β）表达的影响。方法 将80只健康Wistar大鼠按随机区组法分为血管性痴呆模型（VD）组、血管性痴呆模型+丁苯酞氯化钠注射液（NBP+VD）组、假手术+丁苯酞氯化钠注射液（NBP+Sham）组和假手术（Sham）组4个组，每组各20只。采用永久性双侧颈总动脉结扎法制备VD大鼠模型。NBP+Sham组和NBP+VD组大鼠术后1 d开始给予丁苯酞氯化钠注射液腹腔注射，剂量为5 mg/（kg?d），Sham组和VD组大鼠给予生理盐水腹腔注射（0.2 mL/d），各组大鼠均连续腹腔注射给药7 d，每组按时间分为术后1周、2周、4周和8周4个亚组，于不同时间点断头取脑分离海马组织。采用ELISA方法检测海马组织TNF-α和IL-1β的表达。结果 术后1、2、4、8周，VD组大鼠海马组织TNF-α和IL-1β的表达均明显高于Sham组（P 　　[关键词] 丁苯酞；血管性痴呆；海马；肿瘤坏死因子α；白介素1β 　　[中图分类号] R743 [文献标识码] A [文章编号] 1673-7210（2017）01（c）-0012-04 　　[Abstract] Objective To study the effects of 3-n-butylphthalide （NBP） on the expression of TNF-α and IL-1β at different time points in the hippocampus of vascular dementia rats. Methods A total of 80 Wistar rats were assigned to vascular dementia models （VD） group， vascular dementia models + NBP injection （NBP+VD） group， sham surgery + NBP injection （NBP+Sham） group and sham surgery （sham） group by randomized blocks， with 20 rats in each group. Vascular dementia rat model was established by ligating bilateral common carotid artery. The NBP+VD group and NBP+Sham group were intraperitoneally injected with NBP 5 mg/（kg?d）， VD group and Sham group were intraperitoneally injected with 0.2 mL/d saline for 7 days. Each group was divided into four subgroups （n=5） at 1， 2， 4 and 8 weeks after surgery. The rats in each group were decapitated to obtain brains at different time points， and hippocampus were isolated. TNF-α and IL-1β expressions in the hippocampus were determined by ELISA method. Results The expressions of TNF-α and IL-1β in the hippocampus of VD group were significantly higher than those of Sham group at each time point（P 　　1 材料与方法 　　1.1 实验动物、主要试剂和仪器 　　80只健康SPF级Wistar大鼠（雌雄各半），体重200～220 g，购于吉林大学基础医学院动物中心，动物合格证号：SCXK（吉）2003-001。丁苯酞氯化钠注射液（石药集团恩必普药业有限公司，批准文号：国药准字。大鼠TNF-α酶联免疫检测试剂盒和大鼠IL-1β酶联免疫检测试剂盒购自北京绿源博德生物科技有限公司。酶标仪（ELX800，美国Bio-Tek公司）。 　　1.2 实验动物分组 　　大鼠饲养于吉林大学第二医院动物实验室，室内温度22～26℃，相对湿度40%～60%，光