raf―1、mek―2、erk―1在胃癌组织中的表达及其意义.docVIP

Raf―1、MEK―2、ERK―1在胃癌组织中的表达及其意义 　　[摘要] 目的 探?Raf-1、MEK-2、ERK-1在胃癌组织中的表达形式及其意义，并分析此种信号途径与胃癌的临床病理特征之间是否存在联系，并为胃癌抗EGFR靶向治疗的分子标志物筛选提供依据。 方法 通过免疫组化中SP法检测60例胃癌患者标本胃癌组织和正常的胃组织中Raf-1、MEK-2、ERK-1的表达水平并进行比对。 结果 经统计60例胃癌组织当中Raf-1、MEK-2、ERK-1的表达率分别为88.33%（53/60）、85.00%（51/60）、78.33%（47/60）。而正常的胃组织当中Raf-1、MEK-2、ERK-1均未见到阳性表达。被胃癌细胞转移的淋巴结组织中Raf-1、MEK-2、ERK-1的阳性表达率更为显著，分别高达95.12 %（39/41），90.24 %（37/41），87.80 %（36/41）。未见癌细胞转移的淋巴结中Raf-1、MEK-2、ERK-1均无表达。实验证实Raf-1、MEK-2、ERK-1的表达水平与胃癌的临床特征中的肿瘤分化程度、浸润深度、淋巴结转移以及TNM分期有明显相关性（P 　　[关键词] 胃癌；Raf-1；MEK-2；ERK-1；Raf/MEK/ERK；免疫组化 　　[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-9701（2017）15-0030-04 　　[Abstract] Objective To investigate the expression pattern and significance of Raf-1， MEK-2 and ERK-1 in gastric carcinoma and analyze whether there is a link between this signaling pathway and the clinicopathological features of gastric cancer，and to provide the basis for the screening of molecular markers for gastric cancer anti-EGFR targeted therapy. Methods The expression levels of Raf-1， MEK-2 and ERK-1 in gastric cancer tissues and normal gastric tissueswere detected by SP immunohistochemical method and compared in 60 patientswith gastric cancer. Results The expression rates of Raf-1， MEK-2 and ERK-1were 68.33% （53/60）， 85.00% （51/60） and 78.33% （47/60） respectively in 60 cases of gastric cancer. However， the positive expressions of Raf-1， MEK-2 and ERK-1 were not observed in normal gastric tissues. The positive expression rates of Raf-1， MEK-2 and ERK-1 in lymph node metastasis of gastric cancer cells were 95.12%（39/41）， 90.24%（37/41）， 87.80%（36/41）， which were significantly higher. Raf-1， MEK-2 and ERK-1 were not expressed in lymph nodes without lymph node metastasis. The expression levels of Raf-1， MEK-2 and ERK-1 were significantly correlated with the degree of tumor differentiation， depth of invasion，lymph node metastasis and TNM staging in the clinical features of gastric cancer（P 　　[Key words] Gastric cancer； Raf-1； MEK-2； ERK-1； Raf/MEK/ERK； Imm