侧孢短芽孢杆菌A60的筛选及其对辣椒疫霉的室内防-中国农业科学院.PDFVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2017, 33 9 :160-165 侧孢短芽孢杆菌 A60 的筛选及其对辣椒疫霉的室内 防效测定 郝楠  仝赞华  邱德文 （中国农业科学院植物保护研究所，北京 100081） 摘 要 ： 采用平板对峙法对分离自海南43 份土样的700 余株细菌进行筛选，获得一株对辣椒疫霉病菌 （ Phytophthora ）具有高效抑制活性的拮抗菌A60，根据形态特征、生理生化特征和16S rDNA 序列分析鉴定其为侧孢短芽孢杆菌（ capsici Brevibacillus laterosporus ）。分别在辣椒和甜椒上进行室内防效测定，结果表明，加入拮抗菌A60 的辣椒，在第3 天时未发病，对辣椒疫霉病的 防效达100%，第5 天时为95.71%， 第7 天仍可达到92.22% ；加入拮抗菌A60 的甜椒，在第3 天时未发病，防效达100%，第5 天时为75.26%，第7 天仍达73.20%，说明拮抗菌A60 能够明显的降低发病率和病情指数，具有较好的防病持效性。 关键词 ： 辣椒疫霉 ；侧孢短芽孢杆菌 ；生物防治 DOI ：10.13560/ki.biotech.bull.1985.2017-0210 Isolation of Brevibacillus laterosporus A60 and Its Greenhouse Control Efficiency Against Phytophthora capsica HAO Nan TONG Zan-Hua QIU De-Wen （The Institute of Plant Protection ，Chinese Academy of Agricultural Sciences ，Beijing 100081 ） Abstract: A60 strain with high inhibition rate against Phytophthora capsici was screened from 700 strains isolated from 43 soil samples in Hainan province. The A60 strain was identified as Brevibacillus laterosporus based on physiological and biochemical characteristics and a 16S rDNA gene sequence analysis. The control effects of A60 strain against Phytophthora on Capsicum annuum and Capsicum fructescens were determined in greenhouse. The results showed that the biocontrol efficacy of A60 strain on C. annuum L. were 100%，95.71% and 92.22% at 3rd，5th and 7th day，respectively