大孔吸附树脂分离纯化洋葱皮黄酮的研究-河南农业科学.PDF

※工艺技术 食品科学 2011, Vol. 32, No. 12 133 1 1, 2 1 1 徐怀德 ，陈 佳 * ，包 蓉 ，刘 坤 ，李 晋 (1.西北农林科技大学食品科学与工程学院，陕西 杨凌 712100 ；2.西北农林科技大学林学院，陕西 杨凌 712100) 　　 以黄酮含量为指标，比较6 种大孔吸附树脂对洋葱皮黄酮的吸附和解吸效果。通过静态吸附与解吸实验， 筛选出效果较好的X-5 树脂进行动态实验研究。结果表明：X-5 树脂纯化洋葱皮黄酮的工艺条件为吸附流速2mL/min 、 上样溶液pH5.0 、上样液质量浓度0.5mg/mL 、50mL 80% 乙醇作为洗脱液，洗脱流速lmL/min 。经X-5 树脂纯化 后，洋葱皮黄酮纯度从 7.95% 提高到 80.78% 。三次重结晶后其纯度可达94.5% 。该方法简单可行，纯化效果好， 适合于工业化生产。 大孔吸附树脂；分离；纯化；洋葱皮；黄酮 Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption 1 1, 2 1 1 XU Huai-de ，CHEN Jia *，BAO Rong ，LIU Kun ，LI Jin (1. College of Food Science and Engineering, Northwest AF University, Yangling 712100, China； 2. College of Forestry, Northwest AF University, Yangling 712100, China) Abstract ：The characteristics of adsorption and desorption of onion peel flavonoids on six kinds of macroporous resins were compared. Static adsorption and desorption experiments indicated that X-5 resin was the best resin for the separation and purification of flavonoids from onion peel. The optimal process parameters for purifying onion peel flavonoids with X-5 resin were sample loading flow rate of 0.5 mg/mL, sample pH 5.0 and 50 mL of 80% ethanol of as eluent at a flow rate of 1.0 mL/min. After the purification, the purity of flavonoids from onion peel was increased from 7.95% to 80.78% and could reach up to 94.5% after further triple recrystallization. The developed method is simple and feasible for industrialization. Key words ：macroporous adsorption resin ；separation; purification ；onion peel ；flavonoids 中图分类号：TS255 文献标识码