氢气饱和生理盐水对离体大鼠心脏LVEDP和MDA、SOD的影响.docVIP

精品论文 参考文献 氢气饱和生理盐水对离体大鼠心脏LVEDP和MDA、SOD的影响 1.河北大学附属医院 河北保定 071000；2.河北大学化学院 河北保定 071000； 3.河北大学生理教研室 河北保定 071000 摘要：目的 探讨氢气饱和生理盐水对离体大鼠心肌缺血再灌注损伤的保护作用。方法48只大鼠随机分为实验组（24只）和对照组（24只）。对照组采用K-R液，实验组采用K-R液+氢气饱和生理盐水。处理后取出大鼠心脏并建立Langendorff模型，并将离体的心脏按逆灌注10 min，常温旷置20 min、再灌注20 min进行处理。检测丙二醛（malondialdehyde，MDA）及超氧化物歧化酶（superoxide dismutase，SOD）含量，左室舒张末期压（left ventricular end-diastolic pressure，LVEDP）值变化。结果MDA增高以对照组缺血期最显著，且对照组缺血期和缺血再灌注期与实验组同期比较差异有统计学意义（P＜0.01，P＜0.05）。对照组缺血期、缺血再灌注期与缺血前期相比差异明显（P＜0.05）。实验组缺血期、缺血再灌注期与缺血前期无明显差异。SOD活性实验组较对照组增强且具有明显差异（P＜0.01）。对照组缺血再灌注期LVEDP明显增高（P＜0.05），实验组缺血再灌注期LVEDP较缺血前期无显著差异，但与对照组同期比较下降明显（P＜0.05）。结论 氢气饱和生理盐水对心肌缺血再灌注损伤具有明显的保护作用。 关键词：心肌缺血再灌注损伤；氢气饱和生理盐水；MDA；SOD Abstract：Objective To explore the protective effects of hydrogen saturated saline in induced myocardial ischemia reperfusion injury in rats.Methods Forty-eight rats were divided randomly into the experimental group（24）and the control group（24）.The rats of experimental group were treated with K-R solution combined with hydrogen saturated saline，the controls were given K-R solution only.After the treatment，the hearts were excised rats and Langendorff model.And the heart of the in vitro by inverse perfusion for 10 minutes，normal temperature place for 20 minutes，20 minutes of reperfusion.The solution of the cardiac muscle tissue of the rats were treated with saline and then the accounts of malondialdehyde（MDA）and the activity of superoxide dismutase（SOD），and the changes of left ventricular end-diastolic pressure（LVEDP）were determined to compare the different protective effects.Results The accounts of MDA in the control group were the highest，there was a significant difference between the experimental group and the controls concerning with the results of ischemia period and reperfusion period（Plt;0.01，Plt;0.05）.There were differences among the ischemia period and reperfusion period and preischemia period in the control group（Plt;0.05）.In the experimental group，there was not signifi