热缺血再灌注损伤早期大鼠肝脏基因表达谱的实验研究.docVIP

热缺血再灌注损伤早期大鼠肝脏基因表达谱的实验研究 　　【摘要】目的探讨热缺血再灌注损伤（WIRI）早期大鼠肝脏基因表达谱的差异。 　　方法建立SD大鼠肝脏热缺血再灌注损伤模型，取再灌注30 min和再灌注1 h肝组织标本，采用基因表达谱芯片技术检测各组基因表达的变化情况，确定差异表达基因。 　　结果筛选出大鼠肝脏再灌注30 min、再灌注1 h两组共同差异表达基因有77条，其中表达上调56条，表达下调21条。 再灌注30 min或1 h表达上调的基因数均多于表达下调基因数，再灌注1 h表达发生变化的基因总数多于再灌注30 min。 　　?Y论大鼠肝脏热缺血再灌注损伤伴随多基因的表达谱改变，再灌注损伤早期的差异表达基因为研究肝脏WIRI的分子机制提供实验依据。 　　【关键词】肝脏；基因表达；再灌注损伤 　　中图分类号：R657.3文献标识码：ADOI：10.3969/j.issn2017.01.006 　　A experimental study on gene expression profiles in rats liver at early phase of warm ischemia and reperfusion injury 　　[HJ1][HJ] 　　LI Wenchuan1，GAO Liangkui1，LI Haohang1，XU Zuoming1，LUO Zongjiang1，WANG Jianchu2，LU Tao2，PU Jian2▲ 　　（1.Graduate School，2.Department of Hepatobiliary Surgery of Affiliated Hospital，Youjiang Medical University for Nationalities，Baise 533000，China） 　　[HJ2][HJ] 　　【Abstract】ObjectiveTo explore difference of gene expression profiles in rats liver at early phase of warm ischemia and reperfusion injury（WIRI）. 　　MethodsLiver WIRI model of SD rats was established，and liver tissue samples with 30 min and 1 h of reperfusion were taken.Gene expression microarray was used to detect changes of gene expression in each group，so as to identify differential expressed genes（DEGs）. 　　ResultsThe analysis data of the microarray showed that there were 77 common DEGs including 56 upregulated genes and 21 downregulated genes at both 30 min and 1 h of reperfusion.The number of upregulated genes at 30 min or 1 h of reperfusion was larger than that of downregulated genes.And the number of DEGs at 1 h of reperfusion was larger than that 30 min of reperfusion. 　　ConclusionWIRI of rats livers are always complicated with profile changes of multi gene expression，and differential expressed genes at early phase of reperfusion injury can provide experimental basis to molecular mechanism of liver WIRI. 　　【Key words】liver；gene expression；reperfusion injury 　　肝脏热缺血再灌注损伤（warm ischemia and reperfusion injury，WIRI）是肝移植和肝脏切除术中出现的主要临床问题[1]。其主要特征在于肝细胞损伤、无