雌激素论文：绝经期雌鼠舌尖黏膜的病理变化及CK10.CK13的表达变化研究.docVIP

雌激素论文：绝经期雌鼠舌尖黏膜的病理变化及CK10.CK13的表达变化研究 【中文摘要】研究绝经期雌鼠舌尖部黏膜各部位的病理变化及舌黏膜细胞角蛋白（CK）的变化。以判断体内雌激素水平的变化与舌黏膜厚度变化的相关性，为研究灼口症的病因分析奠定理论基础。方法：选择30只8周龄Wistar雌性大鼠随机分2组：选择其中15只施行卵巢摘除术，另15只作为正常对照。9周后处死动物，分别取其舌体组织（舌前1/3），甲醛固定，石蜡包埋，采用HE染色法观察舌尖黏膜各部位的病理变化，并采用免疫组化SABC法分别检测舌背黏膜中细胞角蛋白10、13的表达部位和表达强度。结果: 1、病理学图片显示实验组较对照组大鼠舌尖黏膜各个部位的厚度、形态均有变化：2、CK10与CK13在对照组与去卵巢组大鼠舌尖部舌背黏膜基底上层均有表达，但在去卵巢组其表达明显高于对照组。结论：1、病理图片显示在舌尖区去卵巢组大鼠舌背黏膜较对照组大鼠舌背黏膜的厚度变薄，丝状乳头变短并部分消失，黏膜的角化层变薄且不规则。舌背黏膜的厚度变薄，其耐磨性变差，加上舌尖区的活动度比较大，黏膜薄弱区的敏感性增加。也许这正是发生灼口症的原因所在。舌缘和舌腹的黏膜厚度增加，基底细胞深染，其与灼口症的关联性尚不明确，有待于进一步的研究。2、CK10与CK13的表达上调与雌鼠的雌激素水平下降有关。 【英文摘要】: To study on the pathology change in tongue tip of ovariectomized rat,and toexplore the possible biochemical and topographic variations of CK10 ,CK13 which weredetected in 15 normal rats’ tongue mucosa and in 15 ovariectomized rats?tongue mucosa.Theobjective is to search if there are some relationship between the level of estrogen in the body andthe thickness change of tongue mucosa.Methods: Thirty female Wistar rats were randomly divided into two groups:Choose 15female rats to be ovariectomized rats group and other 15 female rats to be normal group.After9 weeks put them to death，then take the top of their tongue.Put all of them to formaldehydesolution，then paraffin embedding.We use the method of HE to study the change of pathologyin both two groups.And To explore the possible biochemical and topographic variations ofCK10 ,CK13 which were detected in 15 normal rats’ tongue mucosa and in 15 ovariectomizedrats’ tongue mucosa by means of immunohistochemical technique SABC with use of a batteryof monoclonal antibodies against cytokeratins.Results: 1、The photographs of pathology show that there are changes in both thickness andshape in every part of tongue tip of ovariectomized rats. 2、In normal group and inovarectomized group CK10 and CK13 were expressed in the majority in suprabasal cells oftongue mucosa.but it showed extended express of CK10 and CK13 in the OVX group.Conclusions: 1、The photographs of