脉冲电场对宫颈癌hela细胞侵袭转移能力影响的-第三军医大学学报.docVIP

脉冲电场对人宫颈癌Hela细胞侵袭转移能力影响的实验研究 张琴，熊正爱1，李成祥2，姚陈果2 （1重庆医科大学附属第二医院，重庆，400010 2 重庆大学输配电装备与系统安全及新技术国家重点实验室，重庆，40000）： 探讨脉冲电场对人宫颈癌Hela细胞影响。 用固定脉宽100μs、频率1Hz、脉冲数8个，不同的脉冲电场作用于人宫颈癌Hela细胞，Transwell小室进行人工重组基底膜(Matrige1)侵袭和迁移实验，观察脉冲电场对细胞侵袭和转移的影响；采用RT-PCR法检测人宫颈癌Hela细胞中RhoC基因的表达情况。脉冲电场宫颈癌Hela细胞RhoC基因P0.05），且均有剂量依赖关系。结论 脉冲电场宫颈癌Hela细胞RhoC的表达 关键词 脉冲电场；宫颈癌；侵袭 Impact of Pulse Eelectric Fields on Invasion and Metastasis of Human Cervical Cancer Cells Hela Zhang Qin1,Xiong Zheng-ai1,Zhou Wei1, Li Cheng-xiang2, Yao Chen-guo2 the 2nd affiliated hospital of Chongqing Medical University, Chongqing, 400010,China 2. State Key Laboratory of Power Transmission Equipment System Security and New Technology , Chongqing, 400030,China) [Abstract]: Objective To investigate the impact of pulse electric fields on the invasion and metastasis capacity of human cervical cancer Hela cells.Methods The electric fields with permanent duration (100 μs), frequency(1Hz) and pulses(8 pieces) and the electric field intensity from 500v/cm to 1500v/cm was performed on Hela cells, the proliferation and clone capacity of Hela cells is measured with colony formation assay,the invasion and metastasis capacity of Hela cells are evaluated with Transwell chamber,The expression level of mRNAof MMP-2 and RhoC are detected by RT-PCR.Results After being treated with pulse electric fields，the proliferation and clone capacity of Hela cells inhibited(P0.05) .The invasion and metastasis capacity of Hela cells are also inhibited(P0.05)．The expression level of mRNA of MMP-2 and RhoC decreased (P0.05).Above all are dose dependent.Conclusion The pulse electric fields could affect the invasion and metastasis capacity of the human cervical cancer Hela cells．It is probably one of its molecular mechanisms to inhibit the expression of MMP-2 and RhoC． Key Words pulse electric fields; cervical cancer; invasion; metastasis 脉冲电场是生物医学、电工微电子技术等领域治疗肿瘤的新方法。本研究利用不同强度脉冲电场作用于癌Hela细胞，了脉冲电场对宫颈癌Hela细胞RhoC基因mRNA的表达探讨脉冲电场影响肿瘤细胞侵袭转移的可能的机制。 1材料和方法 1.1