笃斯越橘花色苷提取物对光损伤人视网膜色素上皮细胞的保护作用.pdfVIP

324 2010, Vol. 31, No. 21 食品科学 ※营养卫生 宋 雪，韩 勇，籍保平 * ，刘翼翔，吕业春 ( 中国农业大学食品科学与营养工程学院，北京 100083) 目的：通过建立人视网膜色素上皮细胞(hRPE)光损伤模型评价笃斯越橘花色苷对眼睛的保护作用及机理。 方法：光损伤设置光强、光照时间、后续培养时间 3 个影响因素，以细胞存活率和乳酸脱氢酶活性评价模型建立 情况；根据越橘花色苷提取物的干预方式分为预防组、应激组和修复组，每组设高、中、低 3 个浓度，检测细 胞存活率和血管内皮生长因子(VEGF)水平评价保护效果。结果：选择光照度 2500lx ，光照24h 后培养 24h 作为最 佳造模条件，细胞损伤率达 20% ；预防组和应激组能有效保护细胞活力(P ＜0.01) ，修复组没有效果，各组都能 抑制由光损伤诱导 hRPE 细胞 VEGF 的过表达，但是不同浓度之间抑制能力有差异。结论：hRPE 细胞光损伤程度 呈光照强度和光照时间依赖性；越橘花色苷能通过保护 hRPE 的细胞活力和VEGF 的过表达，实现保护眼科健康、 预防眼科疾病的作用。 人视网膜色素上皮细胞；笃斯越橘花色苷提取物；光损伤；血管内皮生长因子 Protective Effect of Bilberry Anthocyanin Extract (BAE) against Photo-oxidative Damage of Human Retinal Pigment Epithelial Cells . . SONG Xue，HAN Yong ，JI Bao-ping* ，LIU Yi-xiang ，LU Ye-chun (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) Abstract ：Objective: To establish a photo-oxidative damage model of human retinal pigment epithelium (hRPE) cells for evaluating the protective effect of bilberry anthocyanin extract (BAE). Methods: Illumination intensity, exposure time and incubation time were selected as the factors to establish the model through evaluating cell variability and LDH activity. Meanwhile, the precaution group, stress group and repairing group were designed to administrate three different concentrations in each group. Cell viability and vascular endothelial growth factor (VEGF) expression level were used to evaluate the preventive effect of BAE. Results: Illumination for 24 h at an intensity of 2500 lx and subsequent incu