甘草质膜水通道蛋白gupip1对甘油的转运.pdfVIP

生物技术通报 # # BIO TE CHN OLOG Y B ULLETIN 20 11 5 GuPIP1 王芳 董乐 戴聪杰 林娈 (, 362000) : 旨在进行甘草质膜水通道蛋白 GuPIP1对水和溶质通透性的鉴定将 GuPIP1 长编码区的 cDNA 构入非洲爪 蟾卵母细胞检测系统的表达载体 psp64 Poly( A ), 并将体外转录获得 GuPIP1的 cRNA显微注射入卵母细胞, 使 GuPIP1基因在 卵母细胞中表达, 通过测定卵母细胞对水和溶质的转运功能来鉴定 GuPIP1的转运功能结果表明, GuPIP1在异源表达系统 非洲爪蟾卵母细胞中具水和甘油通透性, 但不能转运尿素, 为探讨 GuPIP1在植株生理中的作用奠定基础 : 甘草 甘油 水通道蛋白 通透性 Permeability ofChineseLicorice(G ly cyrrh iza ura lensis Fisch) PlasmaMembraneAquaporinGuPIP1 toGlycerol W ang Fang Dong L Dai Congji L in Luan ( S ch ool of Ch em istry and L fi e S c ience, Quanzh ou N orm a l Colleg e, Quanzh ou 362000) Abstrac:t T o x am in th s l ctivity of p lasm a m mbran aquaporin GuPIP1 from Ch in s L icoric , th full l ngth cDNA of GuPIP1w as clon d into th oocyt xpr ssion v ctor psp64 Poly( A ). Com pl m ntary RNA w as synth s iz d in v itro by T7 RNA polym a- ras utilizing th T7 prom ot r of psp64 Poly( A). cRNA w as m icroinj ct d p r oocyt . Th p rm ability to w at r, glyc ro,l and ur a of X enop us oocyt s inj ct d w ith cRNA ncoding GuPIP1w asm asur d. Oocyt s inj ct d w ith cRNA ncod ing rat aquaporin-1( AQP1), rat aquaporin-3( AQP3), and glyc rol transport r( G lpF) w r us d as controls. Functional xpr ssion in X enop us oocyt s r v al d that GuPIP1 can m d iat glyc ral transport in addition to wat r flow. H ow r, GuPIP1 is mi p rm ab l for ur a. Th study provid d a solid foundation for d p r s arch on th phy siologic function ofGuPIP1. K ey w ords: Ch in s L icoric (G ly cyrrh iza uralens is F isch) G lyc rol Aquaporin P rm ab ility