环氧合酶一2基因在膀胱移行细胞癌组织中的表达.docVIP

人膀胱移行细胞癌组织中环氧合酶-2基因的表达及临床意义 张荣贵，张唯力，徐光勇 (重庆医科大学附属第二医院泌尿外科，重庆400010) 提 要：目的：研究环氧合酶-2(Cyclooxygenase-2, COX-2)在膀胱移行细胞癌(Transitional cell carcinoma, TCC)组织中的表达及其意义。方法 采用免疫组化技术，对42例膀胱移行细胞癌和8例正常膀胱组织石蜡标本切片进行免疫组化染色，对阳性表达率与肿瘤分级及侵袭性间的相关性进行分析，逆转录聚合酶链反应(RT-PCR)研究膀胱癌组织中COX-2的表达。结果 COX-2蛋白的阳性表达率与膀胱移行细胞癌分级及侵袭性呈显著相关，在膀胱肿瘤组织中表达率为61.9% (26/42)，COX-2在正常膀胱组织中无表达。在Ⅰ级膀胱移行细胞癌中COX-2蛋白表达率为33.3% (4/12)，在Ⅲ级及Ⅲ级以上膀胱移行细胞癌中COX-2阳性表达率为87.5%(14/16)，Ⅰ级与Ⅲ级及Ⅲ级以上之间有显著差异(P0.05)。COX-2在表浅及浸润膀胱癌中阳性表达率分别为30%和90.9%，统计分析其表达有显著差异(P0.05)；RT-PCR结果显示正常膀胱组织COX-2 mRNA无表达，而膀胱癌组织中可检测出COX-2 mRNA的表达。结论 COX-2在高分级膀胱移行细胞癌和侵袭性膀胱移行细胞癌中阳性表达率显著上升，表明COX-2可能在膀胱移行细胞癌的形成中起重要作用，有可能成为治疗人类膀胱移行细胞癌的作用靶点。 关键词：环氧合酶-2；膀胱癌 Expression of cyclooxygenase-2 in human transitional cell carcinoma of bladder ZHANG Rong-gui，ZHANG Wei-li，XU Guang-yong，HU Zi-li (Department of Urology, the Second Affiliated hospital, Chongqing Medical University，Chongqing 400010，) Abstract：0bjective To study the expression and significance of cyclooxygenase-2 (cox-2)in human transitional cell carcinoma(TCC)of bladder. Methods A total of 42 paraffin-embedded TCC tissue and 8 normal bladder sample were stained by SP immunohistochemical method with specific anti COX-2 antibody．The correlation between the positive expression rates and grades of tumor was analyzed．RT-PCR studied the expression of COX-2 in bladder carcinoma tissue. Results Expression rate of C0X-2 protein was significantly correlated to the tumor grades and their invasiveness．Expression rate of C0X-2 protein in TCC was 61.9%, statistically higher than those in the normal samples (P0.05). Expression of C0X-2 were detected in 33.3% with Low-grade TCC and 87.5% with high-grade TCC(P0.05). Expression of C0X-2 were detected in 30.0% with non-invasive TCC and 90.9% with invasive TCC(P0.05)．RT-PCR showed that the expression of C0X-2 mRNA was strongly up-regulated in bladder carcinoma. Conclusion The expression of C0X-2 is enhanced in high-grade and invasive TCC, suggesting a possible role of C0X-2 in the