双膦酸盐对NFATc1及相关因子p-NF-κBp-c-Jun在破骨细胞分化过程.PDFVIP

　 军 杂 2015年10月1 日　第40卷　第10期 778 解放 医学 志　 论　著 双膦酸盐对NFATc1及相关因子p-NF- κB 、p-c-Jun在破骨细胞 分化过程中的影响 董伟，彭宏峰，梁永强，冯晓洁，廖囡囡，戚孟春 [摘要]　目的　研究双膦酸盐(ALN)对破骨细胞(OC)分化过程中关键因子NFATc1及其信号分子p-NF- κB 、p-c-Jun 的影响。方法　采用小鼠单核巨噬细胞RAW264.7诱导培养OC ，细胞培养于培养皿，分A 组(对照组) 、B组(ALN组) 。 培养第2d采用Western blotting 检测NFATc1 、p-NF- κB 、p-c-Jun 基因表达变化情况，培养第4d 采用免疫荧光方法检测 NFATc1表达，培养第7d检测各组OC生成及数目。采用牙本质磨片接种RAW264.7细胞，分组及处理方法同上述培养皿 培养过程，第9d检测骨吸收功能。结果　两组细胞均有TRAP 阳性多核OC生成，并在牙本质磨片上形成吸收陷窝；但 A 组TRAP 阳性多核细胞数目、吸收陷窝数目及陷窝面积均大于B组。免疫荧光检测NFATc1表达A 组高于B组；Western bl otting检测NFATc1 、p-NF- κB 、p-c-Jun表达B组较A 组低，两组有显著性差异(P 0.01) 。结论　双膦酸盐通过下调p-NF- κB 、p-c-Jun 的表达，最终抑制NFATc1 的表达，从而抑制OC生成和骨吸收功能。 [关键词]　阿仑膦酸盐；破骨细胞；NFATc1 ；NF- κB ；基因，jun [中图分类号]　R329.24　　　　[文献标志码]　A　　　　[文章编号]　0577-7402(2015)10-0778-04 [DOI]　10.11855/j.issn.0577-7402.2015.10.02 Effect of bisphosphonates on NFATc1 and correlators p-NF- κB and p-c-Jun in osteoclast differentiation * DONG Wei, PENG Hong-feng, LIANG Yong-qiang, FENG Xiao-jie, LIAO Nan-nan, QI Meng-chun School of Stomatology, North China University of Science and Technology, Tangshan 063000, China * Corresponding author, E-mail: qimengchun@163.com This work was supported by the National Natural Science Foundation of China and the Natural Science Foundation of Hebei Province (C2011401044) [Abstract]　Objective　To study the effect of alendronate (ALN) on NFATc1 and correlated signaling factors p-NF- κB and p-c-Jun in osteoclast differentiation. Methods　Osteoclasts were inductively cultivated with mouse mononuclear macrophage RAW264.7, and they were divided into 2 groups: group A (control group) and group B (ALN-treated group). The protein expressions of NFATc1, p-NF- κB and p-c-Jun were determined with Western blotting at the 2nd day of cultivation; the expre