酶标仪常见技术和应用.pdfVIP

酶标仪常见技术和应用 尹迪 ©2012 For research use only. Not for use in diagnostic procedures. Trademarks mentioned herein are property of Molecular Devices, LLC or their respective owners. Agenda • 酶标仪工作原理及应用范围 • 光吸收检测技术原理及常见应用 • 荧光检测技术原理、分类及常见应用 • 化学分光技术检测原理及常见应用 • Alpha技术特点 ©2012 For research use only. Not for use in diagnostic procedures. Trademarks mentioned herein are property of Molecular Devices, LLC or their respective owners. 酶标仪工作原理 • 工作原理为一台升级版的分光光度计 1.紫外可见分光光度计 2.可见分光光度计 3.紫外分光光度计 4.荧光分光光度计 5.近红外分光光度计 6.红外分光光度计 7.双光束紫外可见分光光度计 不同物质对 特点波长光 吸收值 ©2012 For research use only. Not for use in diagnostic procedures. Trademarks mentioned herein are property of Molecular Devices, LLC or their respective owners. 酶标仪与分光光度计的不同？ 1:通量上 支持6、12、24、48 、96、384孔板，高端酶标仪可达到3456孔板 2:功能上 单功能酶标仪（吸收光、荧光、化学发光） 多功能酶标仪 ©2012 For research use only. Not for use in diagnostic procedures. Trademarks mentioned herein are property of Molecular Devices, LLC or their respective owners. 酶标仪——多功能微孔板读板机（仪） 学名：微孔读板机（仪）Microplate Reader 俗称：国内俗称酶标仪，因早期主要用于酶联免疫检 测（ELISA），故久之习称为酶标仪 ©2012 For research use only. Not for use in diagnostic procedures. Trademarks mentioned herein are property of Molecular Devices, LLC or their respective owners. 酶标仪能做什么？ 凡是与光信号相关的实验、需要高通量、需要节省试 剂的实验 且可以转移到微孔板中液体物质 都可以考虑使用微孔板检测仪（酶标仪）进行信号检 测！ ©2012 For research use only. Not for use in diagnostic procedures. Trademarks mentioned herein are property of Molecular Devices, L