CT358蛋白在沙眼衣原体感染细胞中的定位及特性分析.pdfVIP

生物化学与生物物理进展 Progress in Biochemistry and Biop hys ics 2009, 36(5): 549~555 Localization and Characterization of Hypothetical Protein CT358 in The Chlamydia trachomatis Infected Cells* 1) 1) 2) 3)** 4)** LI Zhong-Yu , WU Yi-Mou , HUANG Qiu-Lin , WANG Shi-Ping , ZHONG Guang-Ming (1) 42100 1, China; Dep artment of Microbiology and Immunology, Unive rs ity of South China, Hengyang 2)Firs t Hosp ital Affi liated of Univ ers ity of South China , Hengyang 42100 1, China; 3)Institute of Schis tosomiasis Research, X iangya School of Medic ine, Central South University, Changsha 4 10078, China; 4) Univ ers ity of Texas Health Sc ience Center at San A ntonio, TX 78229, USA) Abstract To localize and characterize the hypothetical protein CT358 in the chlamydial infected cells. CT358 gene from the Chlamydia trachomatis (C. trachomatis) serovar D genome was amplified and cloned into the pGEX and pDSRedC 1 vectors. The recombinant plasmid pGEX-CT358 was constructed and expressed as GST fusion proteins. The GST-CT358 fusion protein was used to immunize mice to raise the antibodies, which specifically recognized CT358 without cross-reacting with other unrelated proteins. The antibodies were then used to localize the endogenous CT358 protein and determine the expression pattern in Chlamydial infected cells using an indirect immunofluorescence assay (IFA). Meanwhile, pDSRedC 1-CT358 recombinant plasmid was transfected to HeLa cells