阻断CCL20可以减轻树突状细胞对COPD的影响-中国试验动物学报.DOC

慢阻肺大鼠肺组织中树突状细胞表面因子的表达变化及CCL20抗体的干预作用 孙得胜1,2，欧阳瑶1，顾延会1 （1 遵义医学院附属医院 呼吸一科，遵义 563003； 2 华中科技大学同济医学院附属同济医院 呼吸与危重症医学科，卫生部呼吸系统疾病重点实验室摘 要Wistar大鼠，随机分为健康对照组（10只）、慢阻肺模型组（10只）、CCL20单抗组（10只），用气道内注入脂多糖（共2次）联合烟雾刺激（约28天）的方法诱导慢阻肺模型。在实验初始对单抗组大鼠以CCL20单克隆抗体腹腔注射一次。在第29天取大鼠的肺组织观察病理学改变组 基金项目 国家自然科学基金。 第一作者简介 孙得胜（1980-），男，主治医师，博士研究生，主要研究方向：慢性阻塞性肺疾病的发病机制。E-mail：sun_desheng@163.com。 通信作者简介 欧阳瑶（1965-），女，主任医师，教授，主要研究方向：慢性阻塞性肺疾病的基础与临床。E-mail：ouyangyao116@。 Expression of the surface factors of dendritic cells in the lung tissues of rats with COPD and the effect of CCL20 antibody on it SUN De-sheng1,2, OUYANG Yao1, GU Yan-hui1 (1 Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China 2 Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.) [Abstract] Objective To understand the expression of OX62 and CD83 in the lungs of rats with COPD, and to investigate the effect of the CCL20 monoclonal antibody on it. Methods A total of 30 rats were randomly divided into three groups: normal, model, and CCL20 antibody treated. COPD was induced by cigarette smoke exposure 28 days and LPS solution injection twice. The rats were injected with CCL20 antibody in the last group on the first day. The rats were sacrificed on the 29th day. We investigated the pathomorphology of their lungs by HE staining and evaluated the DC distribution in their lungs by immunohistochemistry. Results The HE staining results of the COPD models are consistent with the typical pathological features of COPD patients, the lung pathology in CCL20 group was significantly attenuated than that in the COPD group. Compared with healthy control group, the OX62+DCs in the COPD model group was significantly increased (P0.05), and the CCL20 group was lower than that in the COPD group (P0.05). The CD83+ DCs of COPD group was