肠致病性大肠杆菌适配体筛选研究-食品安全质量检测学报.PDF

6 12 Vol. 6 No. 12 2015 12 Journal of Food Safety and Quality Dec. , 2015 段 诺, 张田力, 吴世嘉, 王周平* (, , 214122) 摘 要: 目的 , 方法 Whole bacteria-SELEX(), , 15 , , , , 2 (Seq.1 Seq.28) 结果 Seq.1 Seq.28 ( 15%), 45.06±6.797 32.31±6.002 nmol/L结论 SELEX , , 关键词: ; ; Whole bacteria-SELEX ; Selection of an aptamer targeted to Enteropathogenic Escherichia coli * DUAN Nuo, ZHANG Tian-Li, WU Shi-Jia, WANG Zhou-Ping (State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University , Wuxi 214122, China) ABSTRACT: Objective Foodborne diseases caused by foodborne pathogenic bacteria have become a great threat to the global food security. The most common is Enteropathogenic Escherichia.coli (EPEC). Therefore, it will be of great significance to develop rapid and accurate detection method for ensuring food safety and national health. Methods In this work, a whole-bacteria Systemic Evolution of Ligands by Exponential