微藻生物膜营养环境对微藻生长和油脂积累影响.pdfVIP

中国环境科学 2016,36(8) ：2463~2469 China Environmental Science 微藻生物膜营养环境对微藻生长和油脂积累影响 * 熊 伟,黄 云 ,付 乾,钟年丙,朱 恂,廖 强 (1.重庆大学,低品位能源利用技术及教育部重点实验室,重庆 400030 ；2.重庆大学工程热物理研究所,重庆 400030) 摘要：以琼脂凝胶作为微藻营养物质的供给源层,微孔滤膜作为微藻生物膜吸附基质,构建了源层/基质层双层结构光合微藻生物膜反应器, 研究了微藻生物膜营养环境对微藻生长和油脂积累的影响,实验表明:当琼脂浓度从 0.125%(W/ W)升高到 8%时,生物膜含水量从 0.62 减少 到 0.25g,微藻细胞直径从 3.87 减小到 3.35µm,微藻生物膜厚度从 91.23 降低到 62.33µm,单位厚度生物膜内含水量降低 40.49%,CO2 和光向 2 生物膜内部传递阻力减小,为光合反应提供充足碳源和光照,微藻生物膜密度从 40.56 提高了 45.86%至 59.16g/m ,微藻油脂产量提高了 63.39%. 关键词：微藻；琼脂凝胶；生物膜；油脂 中图分类号：X171,TK6 文献标识码：A 文章编号：1000-6923(2016)08-2463-07 * Effect of nutrient solution content of biofilm on algal growth and lipid accumulation. XIONG Wei, HUANG Yun , FU Qian, ZHONG Nian-bing, ZHU Xun, LIAO Qiang (1. Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Ministry of Education, Chongqing University, Chongqing 400030, China；2.Institute of Engineering Thermophysics, Chongqing University, Chongqing 400030, China). China Environmental Science, 2016,36(8)：2463~2469 Abstract ：Agarose gel were coupled with mixed cellulose (CA-CN) membrane to build a photosynthetic microalgal biofilm reactor containing a two-layer source/substrate structure. The agarose gel layer was used to supply nutrient, while the CA-CN membrane layer was used as the framework for biofilm. The effects of nutrient contents on microalgal growth and lipid accumulation were investigated. As the agar powder content in culture increased from 0.125% to 8% (W/ W), the nutrient contents decreased from 0.62 to 0.25g, the cell diameter decreased from 3.87 to3.35µm, the thick