乌司他丁对脓毒症大鼠肺微血管通透性及肺组织PEDF表达影响.docVIP

乌司他丁对脓毒症大鼠肺微血管通透性及肺组织PEDF表达影响 　 作者：蔡施霞，邓烈华，佟琳，孙小聪 【摘要】 目的 观察乌司他丁注射液对脓毒症大鼠肺微血管通透性及肺组织色素上皮衍生因子(PEDF)表达的影响，探讨乌司他丁对脓毒症大鼠肺保护的作用机制。方法 30只健康的雄性SD大鼠随机分为3组：假手术组(A组)、阳性对照组(B组)、乌司他丁治疗组(C组)，每组10例。除假手术组外，其他组均施行盲肠结扎穿孔术(CLP)复制大鼠脓毒症模型，治疗组同时给予乌司他丁腹腔注射(100ku/kg)，术后4h随机处死大鼠5只取组织标本，剩余5只大鼠经颈外静脉注射伊文思蓝观察肺微血管通透性。分别采用逆转录聚合酶链反应(RT-PCR)法和酶联免疫吸附(ELISA)法检测肺组织中PEDF的表达。结果 B组肺微血管通透性明显高于A、C组，C组肺微血管通透性高于A组;B组肺组织PEDF表达水平明显低于A、C组，C组肺组织PEDF表达水平低于A组(Plt;0.05)。结论 乌司他丁能明显抑制脓毒症大鼠肺微血管通透性增高，这种肺保护作用的机制可能与增加PEDF表达有关。 【关键词】 乌司他丁注射液;脓毒症;肺微血管通透性;PEDF 　　【Abstract】 Objective To observe the permeability of pulmonary microvascular and expression of Pigment epithelium-derived factor in septic rats treated with Ulinastatin injection, and to investigate the action mechanism of Ulinastatin in in pulmonary function protect.Methods A total of 30 healthy male SD rats were randomly divided into three groups, and each group has 10 rats,Group A received sham operation and the others underwent cecal ligation and puncture (CLP) operation.All the rats were accepted intraperitoneal injection of normal saline (group A,B) or Ulinastatin (100ku/kg). After four hours, Lung tissue express of PEDF in all rats were detected by reverse transcription-polymerase chain reaction(RT-PCR) and Enzyme-linked immunosorbent assay(ELISA) methods respectively and the permeability of pulmonary vascular were observed by injecting Evans Blue into jugular vein of rats. Results The permeability of pulmonary microvascular in group B were significantly higher than group A and C,which in group C were higher than group A.The expression levels of PEDF in group B were significantly lower than group A and C,which in group C were lower than group A.Conclusions Ulinastatin is a reliable protection agent for septic induce acute lung injury, and its mechanism of action may be involved in the increase of PEDF expression. 　　【Key words】 Ulinastatin injection;Sepsis;Pulmonary microvascular;PEDF 　　脓毒症(sepsis) 是合并感染的全身性炎症反应综合征( systemic inflammation response syndrome, SI