中等强度跑台运动对去卵巢大鼠骨量及相关血清性激素水平影响.docVIP

中等强度跑台运动对去卵巢大鼠骨量及相关血清性激素水平影响 　 作者：卜淑敏 陈永杰 王颖捷 郝利科 【摘要】 目的 观察中等强度跑台运动对去卵巢大鼠骨量和相关血清性激素水平的影响，探讨其预防绝经后骨质疏松发生的作用机制。方法 40只未经产雌性SD大鼠，按体重随机分为假手术、去卵巢静止、去卵巢运动和雌激素4组。去卵巢运动组每周进行4次45 min、速度18 m/min，坡度5°的跑台训练;雌激素组每周颈部皮下注射3次17β雌二醇，每次50 μg/kg体重。结束后，称量腹腔内脂肪和子宫重量;测定血清雌激素(E2)、孕酮(P4) 和睾酮(T)含量;检测右侧游离股骨和胫骨的骨密度(BMD)和骨矿物(BMC) 含量。结果 中等强度跑台运动能显著增加去卵巢大鼠股骨近端、中段和远端以及胫骨近端的BMC、BMD以及血清E2水平，显著降低去卵巢大鼠腹腔内脂肪重量以及血清P4和T水平。结论 中等强度跑台运动抗去卵巢大鼠骨量下降的效应至少部分可能与其对血清性激素水平的调节有关。 【关键词】 运动;去卵巢大鼠;骨密度;性激素 　　【Abstract】 Objective To observe the effects of moderateintensity treadmill exercise on bone mass and related serum sex hormones in ovariectomized rats. Methods Forty 3monthold female SD rats were either shamoperated (SHAM) or ovariectomized (OVX) and randomly divided into four groups by body weight: SHAM, OVX, OVX rats treated with exercise (EX group, 18 m/min, 5% uphill, 45 min/day, 4 times/week), OVX rats treated with 17βestrogen groups (E2 group, 50 μg/kg once, 3 times/w). At the end of the experiment, blood was collected from abdominal vein with general anesthesia. The levels of serum E2, progesterone (P4), and testosterone (T) as well as the BMC and BMD of right femur and tibia were measured. Results The BMC and BMD in all three sites of femur and proximal tibia as well as the serum E2 level were increased remarkably in EX group compared with those of OVX group. However, the abdominal fat weight, the levels of serum P4 and T were decreased significantly while no change was found in uterus index between the OVX group and EX group. Conclusions The antiosteopenia activity of moderate treadmill exercise in OVX rats may be at least partially connected with sex hormones. 　　【Key words】 Exercise; Ovariectomized rats; Bone mineral density; Sex hormones 　 骨质疏松(OP)是一种以骨量减少、骨组织微细结构破坏、骨脆性增加、易发生骨折的慢性骨代谢疾病，目前已成为一种在世界范围内严重威胁老年人健康，尤其是绝经后妇女的常见疾病〔1〕。运动能防治OP已为人们所熟知〔2〕，相关研究表明，中等强度跑台运动能减缓绝经后妇女〔3〕或去卵巢动物〔4〕骨量的丢失，而目前对其作用机制还知之甚少。为此，本研究采用前期建立的去卵巢大鼠模型〔5〕，观察了中等强度跑台运动和17β雌二醇(E2)对去卵巢大鼠骨矿物含量(BMC)、骨密度(BMD)及相关血清性激素水平的影响，旨在为揭示运动预防绝经后骨质