不同中药对豚鼠胆色素结石模型生化学影响.docVIP

不同中药对豚鼠胆色素结石模型生化学影响 　【摘要】 　　目的：观察中药清胆胶囊和养肝利胆颗粒对豚鼠胆囊胆色素结石模型的生化学影响。方法：雄性豚鼠皮下注射林可霉素建立胆色素结石模型，并设立空白组、模型组、清胆胶囊干预组和养肝利胆颗粒干预组，观察各组动物的成石率、胆汁中总胆红素（total bilirubin, TBIL）、游离胆红素（unconjugated bilirubin, UCB）和钙离子浓度等指标的变化。结果：与模型组比较，清胆胶囊组和养肝利胆颗粒组成石率均显著降低（P＜0.01），胆汁中总胆红素、游离胆红素和钙离子浓度也明显降低（P＜0.05，P＜0.01）。结论：清胆胶囊和养肝利胆颗粒可通过降低胆汁中总胆红素、游离胆红素和钙离子含量而逆转成石胆汁，起到预防胆色素结石形成的作用。 【关键词】 利胆剂 胆色素类 胆结石 豚鼠 　　Effects of different Chinese herbal medicines on biochemical parameters in guineapig with pigment gallstones 　　Jingzhe ZHANG, Xuelin ZHANG ,Xiaoqiang LIANG, Honggang GU , Peiting ZHU 　　Department of Hepatobiliary Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China 　　Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China 　　Objective: To observe the effects of Qingdan Capsule (QDC) and Yanggan Lidan Granule (YGLDG), two kinds of compound traditional Chinese herbal medicines, on biochemical parameters in guineapigs with pigment gallstones. 　　Methods: An animal model of pigment gallstones was established in male guineapigs by hypodermic injection of lincomycin. The guineapigs were randomly divided into blank control group, untreated group, QDC group and YGLDG group. There were 8 guineapigs in each group. After tenday treatment, animals were sacrificed and sampled to calculate the rate of stone formation, total bilirubin (TB), unconjugated bilirubin (UCB) and Ca2+ density in bile of the four groups. 　　Results: In comparison with the untreated group, the rates of stone formation in the QDC and YGLDG groups were significantly decreased (Plt;0.01). TBIL, UCB and Ca2+ content of bile in both QDC and YGLD groups was also significantly decreased (Plt;0.05, Plt;0.01). 　　Conclusion: QDC and YGLD have good effects on biochemical changes of animal model of pigment gallstone in reversing the lithogenesity of bile by reducing the content of TB, UCB and Ca2+, hence resulting in clinical treatm