damage associated molecular pattern molecule-induced micrornas (dampmirs) in human peripheral blood mononuclear cells损害相关的分子模式molecule-induced小分子核糖核酸(dampmirs)在人类外周血单核细胞.pdfVIP

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damage associated molecular pattern molecule-induced micrornas (dampmirs) in human peripheral blood mononuclear cells损害相关的分子模式molecule-induced小分子核糖核酸(dampmirs)在人类外周血单核细胞.pdf

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damage associated molecular pattern molecule-induced micrornas (dampmirs) in human peripheral blood mononuclear cells损害相关的分子模式molecule-induced小分子核糖核酸(dampmirs)在人类外周血单核细胞

Damage Associated Molecular Pattern Molecule-Induced microRNAs (DAMPmiRs) in Human Peripheral Blood Mononuclear Cells 1 1 2 4 1 3 Sebnem Unlu *, Siuwah Tang , E na Wang , Ivan Martinez , Daolin Tang , Marco E. Bianchi , 1 1 Herbert J. Zeh, III , Michael T. Lotze * 1 Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, 2 Infectious Disease and Immunogenetics Laboratory, Department of Transfusion Medicine, National Institutes of Health, Bethesda, Maryland, United States of America, 3 Department of Molecular Biology and Functional Genomics, San Raffaele University and Scientific Institute, Milano, Italy, 4 Department of Genetics, School of Medicine, Yale University, New Haven, Connecticut, United States of America Abstract Endogenous damage associated molecular pattern molecules (DAMPs) released from necrotic, damaged or stressed cells are associated with an inflammatory response. Whether the microRNA (miR) expression signature of this response is different from that of a pathogen associated molecular pattern (PAMP)-stimulated inflammatory response is unknown. We report here that miR-34c and miR-214 are significantly expressed in fresh human peripheral blood mononuclear cells (PBMCs) exposed to DAMP-containing freeze-thaw lysates, or to conditioned media from serum-starved and glucose-deprived cells (p,6 61024 and p,3.7 61023), respectively. Interestingly, only miR-34c expression was differentially expressed in PBMCs exposed to freeze-thaw lysates or conditioned media from wildtype High Mobility Group B1 (HMGB1+/+) mouse embryonic fibroblast (MEF) cells, when compared to cultures exposed to lysates or conditi