cord blood leptin levels of healthy neonates are associated with ifn-γ production by cord blood t-cells健康新生儿脐血瘦素水平与脐带血ifn-γ生产的t细胞有关.pdfVIP

Cord Blood Leptin Levels of Healthy Neonates Are Associated with IFN-c Production by Cord Blood T-Cells 1 1. 2. 3 Athanasia Mouzaki *, Ioannis Panagoulias , George Raptis , Evagellia Farri-Kostopoulou 1 Division of Hematology, Department of Internal Medicine, Medical School, University of Patras, Patras, Greece, 2 Royal Oldham Hospital, Department of Child Health, Oldham, United Kingdom, 3 Neonatal Intensive Care Unit, St Andrew’s General Hospital of Patras, Patras, Greece Abstract Leptin is a hormone synthesized by adipocytes and other tissues, including the placenta, and it regulates food intake and energy expenditure, reproductive and immune functions. To investigate the role of leptin in neonatal immunity, we measured serum leptin and cytokine (IFN-c, TNF-a, IL-2, IL-4, IL-10, IL-12) levels in the cord blood (cb) of 510 healthy neonates, 14 small for gestational age (SGA), 312 appropriately grown for gestational age (AGA) and 184 large for gestational age (LGA). Median serum leptin concentration in the whole sample was 11 ng/ml. In 11.2% neonates (1 SGA, 32 AGA, 24 LGA), leptin levels were .90th percentile (median 39 ng/ml). In 33.3% of those (3.72% of total sample) with the highest leptin levels (median 46 ng/ml), significantly elevated levels of serum IFN-c were also found (mean 27.11 pg/ml, range 17.5–38.5 pg/ml). In neonates with leptin levels ,50th percentile (median 12 ng/ml) or ,10th percentile (median 1 ng/ml), serum IFN-c levels were negligible. All other cytokines measured, were , the assays’ detection limits. To investigate whether leptin can independently influence cytokine gene expression by