core gene set as the basis of multilocus sequence analysis of the subclass actinobacteridae核心基因的基础设置为茎actinobacteridae子类的序列分析.pdfVIP

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core gene set as the basis of multilocus sequence analysis of the subclass actinobacteridae核心基因的基础设置为茎actinobacteridae子类的序列分析.pdf

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core gene set as the basis of multilocus sequence analysis of the subclass actinobacteridae核心基因的基础设置为茎actinobacteridae子类的序列分析

Core Gene Set As the Basis of Multilocus Sequence Analysis of the Subclass Actinobacteridae ¨ ´ 1 1 2 2 3 Toıdi Adekambi *, Ray W. Butler , Finnian Hanrahan , Arthur L. Delcher , Michel Drancourt , Thomas M. Shinnick1 1 Mycobacteriology Laboratory Branch, Division of Tuberculosis Elimination, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America, 2 Center for Bioinformatics and Computational Biology, University of Maryland, College Park, Maryland, ´ ´ ´ ´ United States of America, 3 Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, CNRS UMR 6236, IRD 198, IFR 48, Faculte de Medecine, Universite ´ ´ de la Mediterranee, Marseille, France Abstract Comparative genomic sequencing is shedding new light on bacterial identification, taxonomy and phylogeny. An in silico assessment of a core gene set necessary for cellular functioning was made to determine a consensus set of genes that would be useful for the identification, taxonomy and phylogeny of the species belonging to the subclass Actinobacteridae which contained two orders Actinomycetales and Bifidobacteriales. The subclass Actinobacteridae comprised about 85% of the actinobacteria families. The following recommended criteria were used to establish a comprehensive gene set; the gene should (i) be long enough to contain phylogenetically useful information, (ii) not be subject to horizontal gene transfer, (iii) be a single copy (iv) have at least