control of viremia and prevention of aids following immunotherapy of siv-infected macaques with peptide-pulsed blood病毒血症的控制和预防艾滋病与peptide-pulsed siv-infected猕猴的免疫治疗后血.pdfVIP

Control of Viremia and Prevention of AIDS following Immunotherapy of SIV-Infected Macaques with Peptide- Pulsed Blood 1 1 1 1 ˆ 1 Robert De Rose , Caroline S. Fernandez , Miranda Z. Smith , C. Jane Batten , Sheilajen Alcantara , 1 1 1 1 2 3 Vivienne Peut , Erik Rollman , Liyen Loh , Rosemarie D. Mason , Kim Wilson , Matthew G. Law , 1,4¤ 1 Amanda J. Handley , Stephen J. Kent * 1 Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia, 2 National Serology Reference Laboratory, Fitzroy, Victoria, Australia, 3 National Centre for HIV Epidemiology and Clinical Research, University of New South Wales, Sydney, New South Wales, Australia, 4 Opal Therapeutics Pty Ltd, Melbourne, Victoria, Australia Abstract Effective immunotherapies for HIV are needed. Drug therapies are life-long with significant toxicities. Dendritic-cell based immunotherapy approaches are promising but impractical for widespread use. A simple immunotherapy, reinfusing fresh autologous blood cells exposed to overlapping SIV peptides for 1 hour ex vivo, was assessed for the control of SIVmac251 replication in 36 pigtail macaques. An initial set of four immunizations was administered under antiretroviral cover and a booster set of three immunizations administered 6 months later. Vaccinated animals were randomized to receive Gag peptides alone or peptides spanning all nine SIV proteins. High-level, SIV-specific CD4 and CD8 T-cell immunity was induced following immunization, both during antiretroviral cover a