comparative microarray analysis of intestinal lymphocytes following eimeria acervulina, e. maxima, or e. tenella infection in the chicken比较微阵列分析肠道淋巴细胞后,艾美球虫属acervulina,e .最大值或大肠tenella感染的鸡.pdfVIP

Comparative Microarray Analysis of Intestinal Lymphocytes following Eimeria acervulina, E. maxima, or E. tenella Infection in the Chicken 1 1 2 1 1 3 Duk Kyung Kim , Hyun Lillehoj *, Wongi Min , Chul Hong Kim , Myeong Seon Park , Yeong Ho Hong , Erik P. Lillehoj4 1 Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America, 2 College of Veterinary Medicine and Research Institute of Life Science, Gyeongsang National University, Jinju, Korea, 3 Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea, 4 Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, United States of America Abstract Relative expression levels of immune- and non-immune-related mRNAs in chicken intestinal intraepithelial lymphocytes experimentally infected with Eimeria acervulina, E. maxima, or E. tenella were measured using a 10K cDNA microarray. Based on a cutoff of .2.0-fold differential expression compared with uninfected controls, relatively equal numbers of transcripts were altered by the three Eimeria infections at 1, 2, and 3 days post-primary infection. By contrast, E. tenella elicited the greatest number of altered transcripts at 4, 5, and 6 days post-primary infection, and at all time points following secondary infection. When analyzed on the basis of up- or down-regulated transcript levels over the entire 6 day infection periods, approximately equal numbers of up-regulated transcripts were detected following E. tenella primary (1,469) and secondary (1,459) infections, with a greater number of down-regu