biological roles of the podospora anserina mitochondrial lon protease and the importance of its n-domain生物的角色柄孢壳菌属anserina线粒体朗蛋白酶及其n-domain的重要性.pdfVIP

Biological Roles of the Podospora anserina Mitochondrial Lon Protease and the Importance of Its N-Domain ´ 1,2 1,2 ´ 1,2 3 Celine Adam , Marguerite Picard , Michelle Dequard-Chablat , Carole H. Sellem , Sylvie Hermann- 1,2,4 ´ 1,2 Le Denmat *, Veronique Contamine * ´ ´ ´ ´ ´ 1 Univ Paris-Sud, Institut de Genetique et Microbiologie, UMR 8621, Orsay, France, 2 CNRS, Orsay, France, 3 CNRS, Centre de Genetique Moleculaire, UPR 3404, Gif-sur- ´ Yvette, France, 4 Ecole Normale Superieure, Paris, France Abstract Mitochondria have their own ATP-dependent proteases that maintain the functional state of the organelle. All multicellular eukaryotes, including filamentous fungi, possess the same set of mitochondrial proteases, unlike in unicellular yeasts, where ClpXP, one of the two matricial proteases, is absent. Despite the presence of ClpXP in the filamentous fungus Podospora anserina, deletion of the gene encoding the other matricial protease, PaLon1, leads to lethality at high and low temperatures, indicating that PaLON1 plays a main role in protein quality control. Under normal physiological conditions, the PaLon1 deletion is viable but decreases life span. PaLon1 deletion also leads to defects in two steps during development, ascospore germination and sexual reproduction, which suggests that PaLON1 ensures important regulatory functions during fungal development. Mitochondrial Lon proteases are