zic1 is downregulated through promoter hypermethylation, and functions as a tumor suppressor gene in colorectal cancerzic1通过启动子甲基化表达下调和函数作为一个肿瘤抑制基因在结直肠癌.pdfVIP

ZIC1 Is Downregulated through Promoter Hypermethylation, and Functions as a Tumor Suppressor Gene in Colorectal Cancer 1. 2. 1 3 4 4 4 Lihong Gan , Shujie Chen , Jing Zhong , Xian Wang , Emily K. Y. Lam , Xin Liu , Jianbin Zhang , 5 4 2 1 3 Tianhua Zhou , Jun Yu , Jianmin Si , Liangjing Wang *, Hongchuan Jin 1 Department of Gastroenterology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China, 2 Laboratory of Digestive Disease, Clinical Institution of Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China, 3 Key Laboratory of Biotherapy of Zheijang Province, Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China, 4 Department of Medicine and Therapeutics, Institute of Digestive Disease, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR China, 5 Department of Cell Biology and Program in Molecular Cell Biology, School of Medicine Zhejiang University, Hangzhou, China Abstract The transcription factor, Zinc finger of the cerebellum (ZIC1), plays a crucial role in vertebrate development. Recently, ZIC1 has also been found to participate in the progression of human cancers, including medulloblastomas, endometrial cancers, and mesenchymal neoplasms. However, the function of ZIC1 in colon cancer progression has not been defined. In this study, we demonstrate ZIC1 to be silenced or significantly downregulated in colon cancer cell lines. These effects were reversed by demethylation treatment with 5-aza-29-deoxycytidine (Aza). ZIC1 expression is also significantly downregula